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Splicing factor 1 (phospho Ser82) antibody

Anti-Splicing factor 1 (phospho Ser82) antibody used in IHC (Paraffin sections) (IHC-P). GTX55031
Anti-Splicing factor 1 (phospho Ser82) antibody used in Western Blot (WB). GTX55031
Anti-Splicing factor 1 (phospho Ser82) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55031

Cat. No. GTX55031

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399)
See all Splicing factor 1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:1000
ICC/IF 1:100 - 1:500
IHC-P 1:100 - 1:200
Not tested in other applications.

Calculated MW

68 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.42% Potassium Phosphate, 0.87% NaCl, 30% Glycerol

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of Splicing factor 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

splicing factor 1 , BBP , D11S636 , MBBP , ZCCHC25 , ZFM1 , ZNF162

Cellular Localization

Nucleus

Background

This gene encodes a nuclear pre-mRNA splicing factor. The encoded protein specifically recognizes the intron branch point sequence at the 3' splice site, together with the large subunit of U2 auxiliary factor (U2AF), and is required for the early stages of spliceosome assembly. It also plays a role in nuclear pre-mRNA retention and transcriptional repression. The encoded protein contains an N-terminal U2AF ligand motif, a central hnRNP K homology motif and quaking 2 region which bind a key branch-site adenosine within the branch point sequence, a zinc knuckles domain, and a C-terminal proline-rich domain. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2016]

Database

Research Area

DATA IMAGES

Anti-Splicing factor 1 (phospho Ser82) antibody used in IHC (Paraffin sections) (IHC-P). GTX55031

GTX55031 IHC-P Image

IHC-P analysis of formalin fixed human breast cancer tissue section using GTX55031 Splicing factor 1 (phospho Ser82) antibody.
Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)

Anti-Splicing factor 1 (phospho Ser82) antibody used in Western Blot (WB). GTX55031

GTX55031 WB Image

WB analysis of SH-SY5Y (A), Jurkat (B) whole cell lysates using GTX55031 Splicing factor 1 (phospho Ser82) antibody.

Anti-Splicing factor 1 (phospho Ser82) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55031

GTX55031 ICC/IF Image

ICC/IF analysis of formalin-fixed Jurkat cells using GTX55031 Splicing factor 1 (phospho Ser82) antibody.
Red : Primary antibody
Blue : DAPI
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399