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Spred1 antibody

Anti-Spred1 antibody used in Western Blot (WB). GTX85377
Anti-Spred1 antibody used in IHC (Paraffin sections) (IHC-P). GTX85377

Cat. No. GTX85377

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all Spred1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 - 2 μg/mL
IHC-P 2.5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Positive Control

Human Brain Tissue Slide(GTX22203) , Mouse Brain Tissue Lysate(GTX27934)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Spred1 antibody was raised against a 20 amino acid synthetic peptide near the center of the human Spred1.The immunogen is located within amino acids 120 - 170 of Spred1.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

sprouty related EVH1 domain containing 1 , NFLS , PPP1R147 , hSpred1 , spred-1

Cellular Localization

Cell membrane,Nucleus

Background

The Ras-MAP kinase pathway is essential for the differentiation of neuronal cells and myocytes; it is inhibited by Spred1, a member of the Sprouty family of proteins. Spred1 acts by suppressing the phosphorylation and activation of Raf. The Spred proteins have also been implicated in the negative feedback regulation of FGF signaling in embryogenesis and angiogenesis. Further studies have shown that expression levels of Spred1 and Spred2 proteins are inversely correlated with the incidence of tumor invasion and metastasis in human hepatocellular carcinoma (HHC), suggesting that these proteins could be useful as prognostic factors and therapeutic targets in HCC. Defects in this gene are a cause of neurofibromatosis type 1-like syndrome (NFLS). This Spred1 antibody is predicted to have no cross-reactivity to Spred2 or Spred3.

Database

Research Area

DATA IMAGES

Anti-Spred1 antibody used in Western Blot (WB). GTX85377

GTX85377 WB Image

WB analysis of mouse brain tissue lysate using GTX85377 Spred1 antibody.
Working concentration : (A) 1 and (B) 2 μg/ml

Anti-Spred1 antibody used in IHC (Paraffin sections) (IHC-P). GTX85377

GTX85377 IHC-P Image

IHC-P analysis of human brain tissue using GTX85377 Spred1 antibody.
Working concentration : 2.5 μg/ml

REFERENCE

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399