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Src antibody [184Q20]

Anti-Src antibody [184Q20] used in IHC (Paraffin sections) (IHC-P). GTX19336
Anti-Src antibody [184Q20] used in Western Blot (WB). GTX19336
Anti-Src antibody [184Q20] used in IHC (Paraffin sections) (IHC-P). GTX19336

Cat. No. GTX19336

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 184Q20

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse, Rat
Package
50 μg ($399)
See all Src products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1-3 μg/ml
ICC/IF Assay dependent
IHC-P 1:20-1:200
IP Assay dependent
Not tested in other applications.

Calculated MW

60 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA

Preservative

0.1% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human c-Src expressed in E. coli.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

SRC proto-oncogene, non-receptor tyrosine kinase , ASV , SRC1 , THC6 , c-SRC , p60-Src

Cellular Localization

Cell membrane,Mitochondrion inner membrane,Nucleus,Cytoplasm, cytoskeleton

Background

This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Src antibody [184Q20] used in IHC (Paraffin sections) (IHC-P). GTX19336

GTX19336 IHC-P Image

IHC-P analysis of human brain tissue using GTX19336 Src antibody [184Q20].
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:100

Anti-Src antibody [184Q20] used in Western Blot (WB). GTX19336

GTX19336 WB Image

WB analysis of 30 μg of MDA-MB-231 (lane1), MCF 7 (lane2), Jurkat (lane3), A431 (lane4), U2OS (lane5), Hep G2 (lane 6), HEK-293 (lane 7) and HeLa (lane 8) cell lysate using GTX19336 Src antibody [184Q20].
Dilution : 1-3 μg/ml

Anti-Src antibody [184Q20] used in IHC (Paraffin sections) (IHC-P). GTX19336

GTX19336 IHC-P Image

IHC-P analysis of human esophagus tissue using GTX19336 Src antibody [184Q20].
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399