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Src antibody [327]

Cat. No. GTX16885

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 327

Isotype

 IgG1

Application

 WB, IP

Reactivity

 Human, Mouse, Rat
Package
50 μg ($399)
See all Src products

APPLICATION

Application Note

For IP: Use at an assay dependent dilution. For WB: Use at a concentration of 2 -3 μg/ml. Predicted molecular weight: 59.7 kDa. GTX16885 may appear as a doublet on WB due to phosphorylation. This antibody can be used to precipitate active src kinase. Not suitable for IF, IHC-Fr or IHC-P. Optimal dilutions/concentrations should be determined by the researcher.

Calculated MW

61 kDa. ( Note )

Positive Control

COLO-201 cells

Product Note

Reacts with both v-Src and c-Src proteins.

PROPERTIES

Form

Liquid

Buffer

50mM Sodium Phosphate buffer pH7.4, 0.2% Gelatin

Preservative

0.1% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

0.1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Antibody generated by immunizing BALB/c mice with full length native protein (purified) and then fusing with P3X63 Ag8.653 myeloma cells.

Purification

Purified IgG
From hybridoma culture supernatant

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Rous sarcoma oncogene , AW259666 , pp60c-src

Cellular Localization

Cell membrane , Mitochondrion inner membrane , Nucleus , Cytoplasm, cytoskeleton

Database

Research Area

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399