GeneTex
United States (US)

Src antibody [327]

Cat No. GTX16885

Host Mouse
Clonality Monoclonal
Clone Name 327
Isotype IgG1
Application WB, IP
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

For IP: Use at an assay dependent dilution. For WB: Use at a concentration of 2 -3 μg/ml. Predicted molecular weight: 59.7 kDa. GTX16885 may appear as a doublet on WB due to phosphorylation. This antibody can be used to precipitate active src kinase. Not suitable for IF, IHC-Fr or IHC-P. Optimal dilutions/concentrations should be determined by the researcher.

Calculated MW

60 kDa. ( Note )

Positive Control

COLO-201 cells

Specificity/Sensitivity

Reacts with both v-Src and c-Src proteins.
PROPERTIES

Form

Liquid

Buffer

50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.4 with 0.1% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

0.1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Antibody generated by immunizing BALB/c mice with full length native protein (purified) and then fusing with P3X63 Ag8.653 myeloma cells.

Purification

Purified IgG
From hybridoma culture supernatant

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Rous Sarcoma Oncogene,Aw259666,Pp60C-Src,Src

Background

May appear as a doublet due to phosphorylation. May be used to precipitate active Src which can then be used in a kinase reaction.

Database

Research Area

Package List Price ($)
$ 319