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Src antibody [JF0947]

Recombinant antibody
Anti-Src antibody [JF0947] used in Western Blot (WB). GTX01138
Anti-Src antibody [JF0947] used in Western Blot (WB). GTX01138
Anti-Src antibody [JF0947] used in Western Blot (WB). GTX01138
Anti-Src antibody [JF0947] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01138
Anti-Src antibody [JF0947] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01138
Anti-Src antibody [JF0947] used in Flow cytometry (FACS). GTX01138
Anti-Src antibody [JF0947] used in IHC (Paraffin sections) (IHC-P). GTX01138
Anti-Src antibody [JF0947] used in IHC (Paraffin sections) (IHC-P). GTX01138
Anti-Src antibody [JF0947] used in IHC (Paraffin sections) (IHC-P). GTX01138
Anti-Src antibody [JF0947] used in IHC (Paraffin sections) (IHC-P). GTX01138
Anti-Src antibody [JF0947] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01138
Anti-Src antibody [JF0947] used in IHC (Paraffin sections) (IHC-P). GTX01138

Cat No. GTX01138

Host

Rabbit

Clonality

Monoclonal

Clone Name

JF0947

Isotype

IgG

Application

WB, ICC/IF, IHC-P, FACS

Reactivity

Human, Mouse, Rat
Package
100 μl ($369)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:5000
ICC/IF 1:100-1:500
IHC-P 1:50-1:200
FACS 1:50-1:100
Not tested in other applications.

Calculated MW

60 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

1x TBS (pH7.4), 1% BSA, 40% glycerol, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

SRC proto-oncogene, non-receptor tyrosine kinase , ASV , SRC , SRC protooncogene, nonreceptor tyrosine kinase , SRC1 , THC6 , cSRC , p60Src , c Src

Cellular Localization

Cell membrane,Mitochondrion inner membrane,Nucleus,Cytoplasm,Cytoskeleton

Background

This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Src antibody [JF0947] used in Western Blot (WB). GTX01138

GTX01138 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Src antibody [JF0947] (GTX01138) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Src antibody [JF0947] used in Western Blot (WB). GTX01138

GTX01138 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Src antibody [JF0947] (GTX01138) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Src antibody [JF0947] used in Western Blot (WB). GTX01138

GTX01138 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Src antibody [JF0947] (GTX01138) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Src antibody [JF0947] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01138

GTX01138 ICC/IF Image

ICC/IF analysis of PFA-fixed A431 cells using GTX01138 Src antibody [JF0947].
Green : primary antibody
Blue : DAPI
Permeabilization : 0.25% Triton X-100 in PBS

Anti-Src antibody [JF0947] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01138

GTX01138 ICC/IF Image

ICC/IF analysis of PFA-fixed MCF-7 cells using GTX01138 Src antibody [JF0947].
Green : primary antibody
Blue : DAPI
Permeabilization : 0.25% Triton X-100 in PBS

Anti-Src antibody [JF0947] used in Flow cytometry (FACS). GTX01138

GTX01138 FACS Image

FACS analysis of NIH-3T3 cells using GTX01138 Src antibody [JF0947].
Red : primary antibody
Black : unlabelled control
Dilution : 1:50

Anti-Src antibody [JF0947] used in IHC (Paraffin sections) (IHC-P). GTX01138

GTX01138 IHC-P Image

IHC-P analysis of human breast carcinoma tissue section using GTX01138 Src antibody [JF0947].

Anti-Src antibody [JF0947] used in IHC (Paraffin sections) (IHC-P). GTX01138

GTX01138 IHC-P Image

IHC-P analysis of rat brain tissue section using GTX01138 Src antibody [JF0947].

Anti-Src antibody [JF0947] used in IHC (Paraffin sections) (IHC-P). GTX01138

GTX01138 IHC-P Image

IHC-P analysis of human kidney tissue section using GTX01138 Src antibody [JF0947].

Anti-Src antibody [JF0947] used in IHC (Paraffin sections) (IHC-P). GTX01138

GTX01138 IHC-P Image

IHC-P analysis of mouse kidney tissue section using GTX01138 Src antibody [JF0947].

Anti-Src antibody [JF0947] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01138

GTX01138 ICC/IF Image

ICC/IF analysis of PFA-fixed HeLa cells using GTX01138 Src antibody [JF0947].
Green : primary antibody
Blue : DAPI
Permeabilization : 0.25% Triton X-100 in PBS

Anti-Src antibody [JF0947] used in IHC (Paraffin sections) (IHC-P). GTX01138

GTX01138 IHC-P Image

IHC-P analysis of human tonsil tissue section using GTX01138 Src antibody [JF0947].

REFERENCE

There are currently no references for Src antibody [JF0947] (GTX01138). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for Src antibody [JF0947] (GTX01138). Be the first to share your experience with this product.
SDS
Glycerol.pdf
Sodium Azide.pdf