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Src antibody

Anti-Src antibody used in IHC (Paraffin sections) (IHC-P). GTX50504
Anti-Src antibody used in Western Blot (WB). GTX50504

Cat No. GTX50504

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human
Package
100 μl ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:1000
IHC-P 1:50-1:100
Not tested in other applications.

Calculated MW

60 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH 7.4, 150mM NaCl, 0.02% sodium azide, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around aa. 527~531 (P-Q-Y-Q-P) derived from human Src.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

SRC proto-oncogene, non-receptor tyrosine kinase , ASV , SRC1 , THC6 , c-SRC , p60-Src

Cellular Localization

Cell membrane,Mitochondrion inner membrane,Nucleus,Cytoplasm, cytoskeleton

Background

This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Src antibody used in IHC (Paraffin sections) (IHC-P). GTX50504

GTX50504 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX50504 Src antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

Anti-Src antibody used in Western Blot (WB). GTX50504

GTX50504 WB Image

WB analysis of extracts from HT-29, HeLa, and MDA cells using GTX50504 Src antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf