Src (phospho Ser75) antibody

Cat No. GTX32263

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, IHC-P
Reactivity	Human

See all Src products

Datasheet

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:500 - 1:1000
IHC-P	1:100 - 1:200

Not tested in other applications.

Calculated MW

60 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.42% Potassium phosphate, 0.87% Sodium chloride pH 7.3, 30% glycerol, 0.01% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20º C or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of Src. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Src Proto-Oncogene, Non-Receptor Tyrosine Kinase,Asv,Src1,Thc6,C-Src,P60-Src,Src

Cellular Localization

Cell membrane,Cytoplasm,Cytoskeleton,Mitochondrion inner membrane,Nucleus,Perinuclear region

Background

This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Gene ID: 6714
UniProt: P12931

Research Area

DATA IMAGES

GTX32263 IHC-P Image

IHC-P analysis of formalin fixed human brain tissue section using GTX32263 Src (phospho Ser75) antibody.
Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)

GTX32263 WB Image

WB analysis of HeLa (A), HUVEC (B), Jurkat (C), COLO205 (D), EGF-treated HEK293T (E), pervanadate-treated A431 (F) whole cell lysates using GTX32263 Src (phospho Ser75) antibody.