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Src (phospho Tyr416) antibody (Alexa Fluor 488)

Anti-Src (phospho Tyr416) antibody (Alexa Fluor 488) used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54529

Cat No. GTX54529

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human, Mouse, Dog, Chicken
Package
100 μl ($349)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

60 kDa. ( Note )

Product Note

Based on sequence homology, this antibody may cross with other Src family members such as YES1 , FYN proteins when phosphorylated at the equivalent sites.

PROPERTIES

Form

Liquid

Buffer

PBS pH 7.3, 0.2mg/ml BSA, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. Protect from light.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Immunogen

This antibody was produced against a chemically synthesized phosphopeptide derived from the region of Src that contains tyrosine 416. The antibody was affinity purified by sequential epitope-specific chromatography, then conjugated to Alexa Fluor 488. The Tyr418 of human Src is equivalent to Tyr416 of chicken Src.

Purification

Purified IgG

Conjugation

Alexa Fluor 488

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

SRC proto-oncogene, non-receptor tyrosine kinase , ASV , SRC1 , THC6 , c-SRC , p60-Src

Cellular Localization

Cell membrane,Mitochondrion inner membrane,Nucleus,Cytoplasm, cytoskeleton

Background

This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Src (phospho Tyr416) antibody (Alexa Fluor 488) used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54529

GTX54529 ICC/IF Image

ICC/IF analysis of MDCK cells were left untreated (A), stimulated with H2O2 (1 mM for 10 minutes) (B), or pretreated with PP2 prior to H2O2 stimulation (C) using GTX54529 Src (phospho Tyr416) antibody (Alexa Fluor 488).

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf