APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| Assay dependent |
| Assay dependent |
Not tested in other applications.
Calculated MW
Product Note
Based on sequence homology, this antibody may cross with other Src family members such as YES1 , FYN proteins when phosphorylated at the equivalent sites.
PROPERTIES
Form
Liquid
Buffer
PBS pH7.3, 0.2mg/ml BSA
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. Protect from light.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Immunogen
This antibody was produced against a chemically synthesized phosphopeptide derived from the region of Src that contains tyrosine 416. The antibody was affinity purified by sequential epitope-specific chromatography, then conjugated to Alexa Fluor 488. The Tyr418 of human Src is equivalent to Tyr416 of chicken Src.
Purification
Purified IgG
Conjugation
Alexa Fluor 488
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
SRC proto-oncogene, non-receptor tyrosine kinase , ASV , SRC1 , THC6 , c-SRC , p60-Src
Cellular Localization
Cell membrane,Mitochondrion inner membrane,Nucleus,Cytoplasm, cytoskeleton
Background
This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX54529 ICC/IF Image
ICC/IF analysis of MDCK cells were left untreated (A), stimulated with H2O2 (1 mM for 10 minutes) (B), or pretreated with PP2 prior to H2O2 stimulation (C) using GTX54529 Src (phospho Tyr416) antibody (Alexa Fluor 488).
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REFERENCE
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REVIEW
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