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Src (phospho Tyr416) antibody [GT1314]

Recombinant antibody Orthogonal
Anti-Src (phospho Tyr416) antibody [GT1314] used in Western Blot (WB). GTX03226
Anti-Src (phospho Tyr416) antibody [GT1314] used in Western Blot (WB). GTX03226

Cat. No. GTX03226

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 GT1314

Isotype

 IgG

Application

 WB

Reactivity

 Mouse
Package
100 μl ($419)
See all Src products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
Not tested in other applications.

Calculated MW

60 kDa. ( Note )

Product Note

Based on sequence homology, this antibody may cross with other Src family members such as YES1 , FYN proteins when phosphorylated at the equivalent sites.

PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.19 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A phospho specific peptide corresponding to residues surrounding Y419 of human Src. The Tyr419 of human Src (with Met) is equivalent to Tyr416 of chicken Src (without Met).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

SRC proto-oncogene, non-receptor tyrosine kinase , ASV , SRC1 , THC6 , c-SRC , p60-Src

Cellular Localization

Cell membrane , Mitochondrion inner membrane , Nucleus , Cytoplasm, cytoskeleton

Background

This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Src (phospho Tyr416) antibody [GT1314] used in Western Blot (WB). GTX03226

GTX03226 WB Image

Orthogonal

Untreated (–) and treated (+) NIH-3T3 whole cell extracts (15 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Src (phospho Tyr416) antibody [GT1314] (GTX03226) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Src (phospho Tyr416) antibody [GT1314] used in Western Blot (WB). GTX03226

GTX03226 WB Image

Orthogonal

WB analysis of NIH-3T3 cells were treated by pervanadate (1 mM) at 37ºC for 30 minutes after serum-starvation overnight using GTX03226 Src (phospho Tyr419) antibody [GT1314].
Dilution : 1:1000
Loading : 25μg per lane

REFERENCE

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REVIEW

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Package List Price ($)
$ 419