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Src (phospho Tyr416) antibody

Anti-Src (phospho Tyr416) antibody used in Western Blot (WB). GTX24816
Anti-Src (phospho Tyr416) antibody used in IHC (Paraffin sections) (IHC-P). GTX24816

Cat No. GTX24816

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IP, IHC

Reactivity

Human, Mouse, Rat, Yeast, Drosophila, Bovine, Dog, Chicken, Pig, Xenopus
Package
50 μl ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-P 1:10-1:100
IP Assay dependent
IHC Assay dependent
Not tested in other applications.

Calculated MW

60 kDa. ( Note )

Product Note

Based on sequence homology, this antibody may cross with other Src family members such as YES1 , FYN proteins when phosphorylated at the equivalent sites.

PROPERTIES

Form

Liquid

Buffer

PBS pH 7.3, 1mg/ml BSA, 50% glycerol, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide derived from the region of Src that contains tyrosine 416 (tyrosine 417 including the initiating methionine). The sequence is conserved in human, mouse, rat, chicken and frog. The Tyr418 of human Src is equivalent to Tyr416 of chicken Src.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_384107

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

SRC proto-oncogene, non-receptor tyrosine kinase , ASV , SRC1 , THC6 , c-SRC , p60-Src

Cellular Localization

Cell membrane,Mitochondrion inner membrane,Nucleus,Cytoplasm, cytoskeleton

Background

This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Src (phospho Tyr416) antibody used in Western Blot (WB). GTX24816

GTX24816 WB Image

WB analysis of samples using GTX24816 Src (phospho Tyr416) antibody. The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody.

Anti-Src (phospho Tyr416) antibody used in IHC (Paraffin sections) (IHC-P). GTX24816

GTX24816 IHC-P Image

IHC-P analysis of human colon carcinoma tissue using GTX24816 Src (phospho Tyr416) antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:20

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf