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Src (phospho Tyr416) antibody

Anti-Src (phospho Tyr416) antibody used in Western Blot (WB). GTX54701

Cat. No. GTX54701

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human
Package
100 μl ($399)
See all Src products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
Not tested in other applications.

Calculated MW

60 kDa. ( Note )

Product Note

Based on sequence homology, this antibody may cross with other Src family members such as YES1 , FYN proteins when phosphorylated at the equivalent sites.

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A phospho specific peptide corresponding to residues surrounding Y418 of human SRC. The Tyr418 of human Src is equivalent to Tyr416 of chicken Src.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

SRC proto-oncogene, non-receptor tyrosine kinase , ASV , SRC1 , THC6 , c-SRC , p60-Src

Cellular Localization

Cell membrane,Mitochondrion inner membrane,Nucleus,Cytoplasm, cytoskeleton

Background

This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Src (phospho Tyr416) antibody used in Western Blot (WB). GTX54701

GTX54701 WB Image

WB analysis of COLO205 cell lysate using GTX54701 Src (phospho Tyr416) antibody.

REFERENCE

Application Reference

Chuang FK et al. Int J Mol Sci 2020; 21 (11) : 4050 Antiviral Activity of Compound L3 against Dengue and Zika Viruses In Vitro and In Vivo
Application : WB
Chang YX et al. Cancers (Basel) 2020; 12 (12) Chaperonin-Containing TCP-1 Promotes Cancer Chemoresistance and Metastasis through the AKT-GSK3β-β-Catenin and XIAP-Survivin Pathways.
Application : WB
Reactivity : Human
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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399