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Src (phospho Tyr527) antibody

Anti-Src (phospho Tyr527) antibody used in Western Blot (WB). GTX133473
Anti-Src (phospho Tyr527) antibody used in Western Blot (WB). GTX133473
Anti-Src (phospho Tyr527) antibody used in Western Blot (WB). GTX133473
Anti-Src (phospho Tyr527) antibody used in Western Blot (WB). GTX133473

Cat No. GTX133473

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

60 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , NIH-3T3 CIP , NIH-3T3 , NIH-3T3 (starvation for 16 hr) , PC-12 , Rat2

Product Note

Based on sequence homology, this antibody may cross with other Src family members such as YES1 , FYN proteins when phosphorylated at the equivalent sites.

Predict Reactivity

Zebrafish, Bovine, Chicken, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.18 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Src (phospho Tyr527). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2887002

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

SRC proto-oncogene, non-receptor tyrosine kinase , ASV , SRC1 , THC6 , c-SRC , p60-Src

Background

This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Src (phospho Tyr527) antibody used in Western Blot (WB). GTX133473

GTX133473 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Src (phospho Tyr527) antibody (GTX133473) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Src (phospho Tyr527) antibody used in Western Blot (WB). GTX133473

GTX133473 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Src (phospho Tyr527) antibody (GTX133473) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Src (phospho Tyr527) antibody used in Western Blot (WB). GTX133473

GTX133473 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Src (phospho Tyr529) antibody (GTX133473) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Src (phospho Tyr527) antibody used in Western Blot (WB). GTX133473

GTX133473 WB Image

Untreated (–) and treated (+) NIH-3T3 whole cell extracts (20 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Src (phospho Tyr527) antibody (GTX133473) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf