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Src (phospho Tyr527) antibody

Anti-Src (phospho Tyr527) antibody used in IHC (Paraffin sections) (IHC-P). GTX32265
Anti-Src (phospho Tyr527) antibody used in Western Blot (WB). GTX32265

Cat No. GTX32265

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($319)
See all Src products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:1000
IHC-P 1:100 - 1:200
Not tested in other applications.

Calculated MW

60 kDa. ( Note )

Specificity/Sensitivity

Based on sequence homology, this antibody may cross with other Src family members such as YES1 , FYN proteins when phosphorylated at the equivalent sites.

PROPERTIES

Form

Liquid

Buffer

0.42% Potassium phosphate, 0.87% Sodium chloride pH 7.3, 30% glycerol, 0.01% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20º C or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of Src. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

SRC proto-oncogene, non-receptor tyrosine kinase , ASV , SRC1 , THC6 , c-SRC , p60-Src

Cellular Localization

Cell membrane,Mitochondrion inner membrane,Nucleus,Cytoplasm, cytoskeleton

Background

This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Src (phospho Tyr527) antibody used in IHC (Paraffin sections) (IHC-P). GTX32265

GTX32265 IHC-P Image

IHC-P analysis of formalin fixed human breast cancer tissue section using GTX32265 Src (phospho Tyr527) antibody.
Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)

Anti-Src (phospho Tyr527) antibody used in Western Blot (WB). GTX32265

GTX32265 WB Image

WB analysis of K562 (A), HUVEC (B), Jurkat (C), COLO205 (D), EGF-treated HEK293T (E), pervanadate-treated A431 (F) whole cell lysates using GTX32265 Src (phospho Tyr527) antibody.

REFERENCE

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SDS
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 319