*Optimal dilutions/concentrations should be determined by the researcher.
|1:500 - 1:1000
|1:50 - 1:100
Not tested in other applications.
Based on sequence homology, this antibody may cross with other Src family members such as YES1 , FYN proteins when phosphorylated at the equivalent sites.
Mouse, Rat(>80% identity)
PBS (without Mg²⁺ and Ca²⁺) pH 7.4, 150mM NaCl, 0.02% sodium azide, 50% glycerol
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Batch dependent (Please refer to the vial label for the specific concentration.)
The antiserum was produced against synthesized phosphopeptide derived from human Src around the phosphorylation site of tyrosine 529 (P-Q-Yp-Q-P).
Purified by sequential chromatography on phospho- and non-phospho-peptide affinity columns.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
ASV,SRC,SRC protooncogene, nonreceptor tyrosine kinase,SRC1,THC6,cSRC,p60Src,c Src,SRC proto-oncogene, non-receptor tyrosine kinase
Cell membrane,Mitochondrion inner membrane,Nucleus,Cytoplasm,Cytoskeleton
This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
GTX78941 IHC-P Image
IHC-P analysis of human breast carcinoma tissue using GTX78941 Src (phospho Tyr527) antibody.
GTX78941 WB Image
WB analysis of EGF-treated 293 cell lysate using GTX78941 Src (phospho Tyr527) antibody.