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Src (phospho Tyr527) antibody

Anti-Src (phospho Tyr527) antibody used in IHC (Paraffin sections) (IHC-P). GTX78941
Anti-Src (phospho Tyr527) antibody used in Western Blot (WB). GTX78941

Cat No. GTX78941

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human
Package
100 μg ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:1000
IHC-P 1:50 - 1:100
Not tested in other applications.

Calculated MW

60 kDa. ( Note )

Product Note

Based on sequence homology, this antibody may cross with other Src family members such as YES1 , FYN proteins when phosphorylated at the equivalent sites.

Predict Reactivity

Mouse, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH 7.4, 150mM NaCl, 0.02% sodium azide, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized phosphopeptide derived from human Src around the phosphorylation site of tyrosine 529 (P-Q-Yp-Q-P).

Purification

Purified by sequential chromatography on phospho- and non-phospho-peptide affinity columns.
From serum

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

ASV,SRC,SRC protooncogene, nonreceptor tyrosine kinase,SRC1,THC6,cSRC,p60Src,c Src,SRC proto-oncogene, non-receptor tyrosine kinase

Cellular Localization

Cell membrane,Mitochondrion inner membrane,Nucleus,Cytoplasm,Cytoskeleton

Background

This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Src (phospho Tyr527) antibody used in IHC (Paraffin sections) (IHC-P). GTX78941

GTX78941 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX78941 Src (phospho Tyr527) antibody.

Anti-Src (phospho Tyr527) antibody used in Western Blot (WB). GTX78941

GTX78941 WB Image

WB analysis of EGF-treated 293 cell lysate using GTX78941 Src (phospho Tyr527) antibody.

REFERENCE

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REVIEW

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