APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500 - 1:1000 |
| 1:50 - 1:100 |
Not tested in other applications.
Calculated MW
Product Note
This antibody is raised against human Src phosphorylated at Tyr530. Based on sequence homology, it is predicted to react with chicken Src phosphorylated at Tyr527, and it may cross with other Src family members such as YES1 and FYN proteins when phosphorylated at the equivalent sites.
Predict Reactivity
Mouse, Rat(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 150mM NaCl, 50% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human Src around the phosphorylation site of tyrosine 529 (P-Q-Y(p) -Q-P). The Tyr529 of human Src is equivalent to Tyr527 of chicken Src.
Purification
Purified by sequential chromatography on phospho- and non-phospho-peptide affinity columns.
From serum
Conjugation
Unconjugated
RRID
AB_11171463
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
ASV,SRC,SRC protooncogene, nonreceptor tyrosine kinase,SRC1,THC6,cSRC,p60Src,c Src,SRC proto-oncogene, non-receptor tyrosine kinase
Cellular Localization
Cell membrane,Mitochondrion inner membrane,Nucleus,Cytoplasm,Cytoskeleton
Background
This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX78941 IHC-P Image
IHC-P analysis of human breast carcinoma tissue using GTX78941 Src (phospho Tyr527) antibody.
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GTX78941 WB Image
WB analysis of EGF-treated 293 cell lysate using GTX78941 Src (phospho Tyr527) antibody.
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REFERENCE
There are currently no references for Src (phospho Tyr527) antibody (GTX78941). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Src (phospho Tyr527) antibody (GTX78941). Be the first to share your experience with this product.
