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StAR antibody [HL1360]

Recombinant antibody Orthogonal Overexpression
Anti-StAR antibody [HL1360] used in IHC (Paraffin sections) (IHC-P). GTX636800
Anti-StAR antibody [HL1360] used in Western Blot (WB). GTX636800
Anti-StAR antibody [HL1360] used in IHC (Paraffin sections) (IHC-P). GTX636800
Anti-StAR antibody [HL1360] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636800
Anti-StAR antibody [HL1360] used in Western Blot (WB). GTX636800
Anti-StAR antibody [HL1360] used in IHC (Paraffin sections) (IHC-P). GTX636800
Anti-StAR antibody [HL1360] used in Western Blot (WB). GTX636800
Anti-StAR antibody [HL1360] used in IHC (Paraffin sections) (IHC-P). GTX636800

Cat. No. GTX636800

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1360

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Cat, Dog
Package
100 μl ($399),
25 μl ($169)
See all StAR products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

32 kDa. ( Note )

Predict Reactivity

Rat, Sheep, Bovine, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human StAR. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

steroidogenic acute regulatory protein , STARD1

Cellular Localization

Mitochondrion

Background

The protein encoded by this gene plays a key role in the acute regulation of steroid hormone synthesis by enhancing the conversion of cholesterol into pregnenolone. This protein permits the cleavage of cholesterol into pregnenolone by mediating the transport of cholesterol from the outer mitochondrial membrane to the inner mitochondrial membrane. Mutations in this gene are a cause of congenital lipoid adrenal hyperplasia (CLAH), also called lipoid CAH. A pseudogene of this gene is located on chromosome 13. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-StAR antibody [HL1360] used in IHC (Paraffin sections) (IHC-P). GTX636800

GTX636800 IHC-P Image

StAR antibody [HL1360] detects StAR protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded dog kidney.
StAR stained by StAR antibody [HL1360] (GTX636800) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-StAR antibody [HL1360] used in Western Blot (WB). GTX636800

GTX636800 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with StAR antibody [HL1360] (GTX636800) diluted at 1:200000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-StAR antibody [HL1360] used in IHC (Paraffin sections) (IHC-P). GTX636800

GTX636800 IHC-P Image

StAR antibody [HL1360] detects StAR protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
StAR stained by StAR antibody [HL1360] (GTX636800) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-StAR antibody [HL1360] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636800

GTX636800 ICC/IF Image

StAR antibody [HL1360] detects StAR protein by immunofluorescent analysis.
Sample: K562 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: StAR stained by StAR antibody [HL1360] (GTX636800) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-StAR antibody [HL1360] used in Western Blot (WB). GTX636800

GTX636800 WB Image

Various tissue extracts (50 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with StAR antibody [HL1360] (GTX636800) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-StAR antibody [HL1360] used in IHC (Paraffin sections) (IHC-P). GTX636800

GTX636800 IHC-P Image

StAR antibody [HL1360] detects StAR protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded mouse adrenal gland.
StAR stained by StAR antibody [HL1360] (GTX636800) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-StAR antibody [HL1360] used in Western Blot (WB). GTX636800

GTX636800 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with StAR antibody [HL1360] (GTX636800) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-StAR antibody [HL1360] used in IHC (Paraffin sections) (IHC-P). GTX636800

GTX636800 IHC-P Image

StAR antibody [HL1360] detects StAR protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded cat kidney.
StAR stained by StAR antibody [HL1360] (GTX636800) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations