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StAR antibody

Anti-StAR antibody used in Western Blot (WB). GTX105716
Anti-StAR antibody used in Western Blot (WB). GTX105716
Anti-StAR antibody used in Western Blot (WB). GTX105716
Anti-StAR antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105716
Anti-StAR antibody used in IHC (Paraffin sections) (IHC-P). GTX105716

Cat No. GTX105716

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($379),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:100-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

32 kDa. ( Note )

Positive Control

K562 , mouse kidney , STAR-transfected 293T

Predict Reactivity

Rat, Sheep, Bovine, Cat, Dog, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS pH7, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human StAR. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2038046

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

steroidogenic acute regulatory protein , STARD1

Cellular Localization

Mitochondrion

Background

The protein encoded by this gene plays a key role in the acute regulation of steroid hormone synthesis by enhancing the conversion of cholesterol into pregnenolone. This protein permits the cleavage of cholesterol into pregnenolone by mediating the transport of cholesterol from the outer mitochondrial membrane to the inner mitochondrial membrane. Mutations in this gene are a cause of congenital lipoid adrenal hyperplasia (CLAH), also called lipoid CAH. A pseudogene of this gene is located on chromosome 13. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-StAR antibody used in Western Blot (WB). GTX105716

GTX105716 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with StAR antibody (GTX105716) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-StAR antibody used in Western Blot (WB). GTX105716

GTX105716 WB Image

Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with StAR antibody (GTX105716) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-StAR antibody used in Western Blot (WB). GTX105716

GTX105716 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with StAR antibody (GTX105716) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-StAR antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105716

GTX105716 ICC/IF Image

StAR antibody detects StAR protein at mitochondria by immunofluorescent analysis.
Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: StAR protein stained by StAR antibody (GTX105716) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-StAR antibody used in IHC (Paraffin sections) (IHC-P). GTX105716

GTX105716 IHC-P Image

StAR antibody detects StAR protein at cytosol on human hepatoma by immunohistochemical analysis.
Sample: Paraffin-embedded hepatoma.
StAR antibody (GTX105716) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 379
$ 169