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StAR antibody

Orthogonal Overexpression
Anti-StAR antibody used in Western Blot (WB). GTX105716
Anti-StAR antibody used in Western Blot (WB). GTX105716
Anti-StAR antibody used in Western Blot (WB). GTX105716
Anti-StAR antibody used in IHC (Paraffin sections) (IHC-P). GTX105716
Anti-StAR antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105716

Cat. No. GTX105716

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all StAR products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:100-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

32 kDa. ( Note )

Positive Control

STAR-transfected 293T

Predict Reactivity

Rat, Sheep, Bovine, Cat, Dog, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human StAR. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2038046

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

steroidogenic acute regulatory protein , STARD1

Cellular Localization

Mitochondrion

Background

The protein encoded by this gene plays a key role in the acute regulation of steroid hormone synthesis by enhancing the conversion of cholesterol into pregnenolone. This protein permits the cleavage of cholesterol into pregnenolone by mediating the transport of cholesterol from the outer mitochondrial membrane to the inner mitochondrial membrane. Mutations in this gene are a cause of congenital lipoid adrenal hyperplasia (CLAH), also called lipoid CAH. A pseudogene of this gene is located on chromosome 13. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-StAR antibody used in Western Blot (WB). GTX105716

GTX105716 WB Image

Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with StAR antibody (GTX105716) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-StAR antibody used in Western Blot (WB). GTX105716

GTX105716 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with StAR antibody (GTX105716) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-StAR antibody used in Western Blot (WB). GTX105716

GTX105716 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with StAR antibody (GTX105716) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-StAR antibody used in IHC (Paraffin sections) (IHC-P). GTX105716

GTX105716 IHC-P Image

StAR antibody detects StAR protein at cytosol on human hepatoma by immunohistochemical analysis.
Sample: Paraffin-embedded hepatoma.
StAR antibody (GTX105716) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-StAR antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105716

GTX105716 ICC/IF Image

StAR antibody detects StAR protein at mitochondria by immunofluorescent analysis.
Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: StAR protein stained by StAR antibody (GTX105716) diluted at 1:500.
Blue: Hoechst 33342 staining.

REFERENCE

Application Reference

Lai TH et al. J Reprod Immunol 2021; 145 : 103311 TGFβ1 induces in-vitro and ex-vivo angiogenesis through VEGF production in human ovarian follicular fluid-derived granulosa cells during in-vitro fertilization cycle.
Feng X et al. Mol Cell Endocrinol 2021; 519 : 111039 Transplantation of encapsulated human Leydig-like cells: A novel option for the treatment of testosterone deficiency.
Application : ICC/IF
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169