GeneTex
United States (US)

Stanniocalcin 2 antibody

GTX115113_40947_20150903_WB_M_kidney_18121410_976.jpg
GTX115113_40947_IFA_18121410_297.jpg
GTX115113_40947_WB_18121410_474.jpg

Cat No. GTX115113

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

33 kDa. ( Note )

Positive Control

A549 , H1299 , MCF-7 , Mouse kidney

Predict Reactivity

Rat, Bovine, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Stanniocalcin 2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Stanniocalcin 2,Stc-2,Stcrp,Stc2

Cellular Localization

Secreted

Background

This gene encodes a secreted, homodimeric glycoprotein that is expressed in a wide variety of tissues and may have autocrine or paracrine functions. The encoded protein has 10 of its 15 cysteine residues conserved among stanniocalcin family members and is phosphorylated by casein kinase 2 exclusively on its serine residues. Its C-terminus contains a cluster of histidine residues which may interact with metal ions. The protein may play a role in the regulation of renal and intestinal calcium and phosphate transport, cell metabolism, or cellular calcium/phosphate homeostasis. Constitutive overexpression of human stanniocalcin 2 in mice resulted in pre- and postnatal growth restriction, reduced bone and skeletal muscle growth, and organomegaly. Expression of this gene is induced by estrogen and altered in some breast cancers. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX115113_40947_20150903_WB_M_kidney_18121410_976.jpg

GTX115113 WB Image

Stanniocalcin 2 antibody detects Stanniocalcin 2 protein by western blot analysis. Mouse tissue extracts (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Stanniocalcin 2 antibody (GTX115113) diluted at 1:1000.

GTX115113_40947_IFA_18121410_297.jpg

GTX115113 ICC/IF Image

Stanniocalcin 2 antibody detects Stanniocalcin 2 protein at cytoplasm by immunofluorescent analysis.
Sample: MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Stanniocalcin 2 protein stained by Stanniocalcin 2 antibody (GTX115113) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

GTX115113_40947_WB_18121410_474.jpg

GTX115113 WB Image

Sample (30 ug of whole cell lysate)
A: A549
B: H1299
C: MCF-7
12% SDS PAGE
GTX115113 diluted at 1:1000

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169