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Stathmin 1 antibody

KO/KD
Anti-Stathmin 1 antibody used in Western Blot (WB). GTX104707
Anti-Stathmin 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104707
Anti-Stathmin 1 antibody used in Western Blot (WB). GTX104707
Anti-Stathmin 1 antibody used in Western Blot (WB). GTX104707
Anti-Stathmin 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104707
Anti-Stathmin 1 antibody used in Western Blot (WB). GTX104707
Anti-Stathmin 1 antibody used in Western Blot (WB). GTX104707
Anti-Stathmin 1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX104707
Anti-Stathmin 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX104707

Cat. No. GTX104707

Reference 5

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Stathmin 1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

17 kDa. ( Note )

Positive Control

PC-12 , Rat2 , 293T , A431 , H1299 , mouse brain , rat brain , HeLa

Predict Reactivity

Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Stathmin 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1241361

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

stathmin 1 , C1orf215 , LAP18 , Lag , OP18 , PP17 , PP19 , PR22 , SMN

Cellular Localization

Membrane

Background

This gene belongs to the stathmin family of genes. It encodes a ubiquitous cytosolic phosphoprotein proposed to function as an intracellular relay integrating regulatory signals of the cellular environment. The encoded protein is involved in the regulation of the microtubule filament system by destabilizing microtubules. It prevents assembly and promotes disassembly of microtubules. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Stathmin 1 antibody used in Western Blot (WB). GTX104707

GTX104707 WB Image

KO/KD

Wild-type (WT) and Stathmin 1 knockout (KO) HeLa cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Stathmin 1 antibody (GTX104707) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Stathmin 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104707

GTX104707 ICC/IF Image

Stathmin 1 antibody detects Stathmin 1 protein at cytoplasm by immunofluorescent analysis.
Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Stathmin 1 protein stained by Stathmin 1 antibody (GTX104707) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Anti-Stathmin 1 antibody used in Western Blot (WB). GTX104707

GTX104707 WB Image

Stathmin 1 antibody detects STMN1 protein by Western blot analysis.
A. 50 μg mouse brain lysate/extract
12 % SDS-PAGE
Stathmin 1 antibody (GTX104707) dilution: 1:10000

Anti-Stathmin 1 antibody used in Western Blot (WB). GTX104707

GTX104707 WB Image

Various tissue extracts (50 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Stathmin 1 antibody (GTX104707) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Stathmin 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104707

GTX104707 ICC/IF Image

Stathmin 1 antibody detects Stathmin 1 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Stathmin 1 protein stained by Stathmin 1 antibody (GTX104707) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] (GTX11304) diluted at 1:10000.
Blue: Hoechst 33342 staining.
Anti-Stathmin 1 antibody used in Western Blot (WB). GTX104707

GTX104707 WB Image

Sample(30 μg of whole cell lysate)
A:293T
B:A431(GTX27909)
C:H1299
12% SDS PAGE
GTX104707 diluted at 1:3000

Anti-Stathmin 1 antibody used in Western Blot (WB). GTX104707

GTX104707 WB Image

Stathmin 1 antibody detects STMN1 protein by Western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
B. 30 μg Rat2 whole cell lysate/extract
12 % SDS-PAGE
Stathmin 1 antibody (GTX104707) dilution: 1:2000

Anti-Stathmin 1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX104707

GTX104707 IHC-Fr Image

Stathmin 1 antibody detects Stathmin 1 protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: Stathmin 1 protein stained by Stathmin 1 antibody (GTX104707) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-Stathmin 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX104707

GTX104707 IHC-P Image

Stathmin 1 antibody detects Stathmin 1 protein at cytosol on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
Stathmin 1 antibody (GTX104707) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

B?rez-L?pez S et al. Mol Cell Proteomics 2023; 22 (5) : 100544 Translational and Posttranslational Dynamics in a Model Peptidergic System.
Wang SJ et al. J Pers Med 2021; 11 (5) A Bioinformatics Analysis Identifies the Telomerase Inhibitor MST-312 for Treating High-STMN1-Expressing Hepatocellular Carcinoma.
Application : WB
Reactivity : Human
Chou HC et al. Life Sci 2018; (Epub) Proteomic analysis of honokiol-induced cytotoxicity in thyroid cancer cells.
Application : WB
Reactivity : Human
Chang SJ et al. Arch Biochem Biophys 2018; 647 : 10-32 Proteomic investigating the cooperative lethal effect of EGFR and MDM2 inhibitors on ovarian carcinoma.
Application : WB
Reactivity : Human
Lin LH et al. Electrophoresis 2014; Biomarker discovery for neuroendocrine cervical cancer.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169