GeneTex
United States (US)

Stathmin 1 antibody

KO/KD
GTX104707_39694_20170330_WB_KO_watermark_19011506_669.jpg
GTX104707_39694_20150410_IFA_2_19011506_540.jpg
GTX104707_39694_WB_M_brain_19011506_153.jpg
GTX104707_39694_20170929_WB_M_R_19011506_344.jpg
GTX104707_39694_20170807_IHC-Fr_M_19011506_834.jpg
GTX104707_39694_WB_R_19011506_481.jpg
GTX104707_39694_20150410_IFA_19011506_246.jpg
GTX104707_39694_IHC_19011506_961.jpg
GTX104707_WB_19011506_439.jpg

Cat No. GTX104707

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IHC-Fr
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

17 kDa. ( Note )

Positive Control

HeLa , 293T , A431 , H1299 , mouse brain , rat brain , PC-12 , Rat2

Predict Reactivity

Bovine, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Stathmin 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Stathmin 1,C1Orf215,Lap18,Lag,Op18,Pp17,Pp19,Pr22,Smn,Stmn1

Cellular Localization

Membrane

Background

This gene belongs to the stathmin family of genes. It encodes a ubiquitous cytosolic phosphoprotein proposed to function as an intracellular relay integrating regulatory signals of the cellular environment. The encoded protein is involved in the regulation of the microtubule filament system by destabilizing microtubules. It prevents assembly and promotes disassembly of microtubules. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX104707_39694_20170330_WB_KO_watermark_19011506_669.jpg

GTX104707 WB Image

KO/KD

Wild-type (WT) and Stathmin 1 knockout (KO) HeLa cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with Stathmin 1 antibody (GTX104707) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
CRISPR knockout cell lines generated by www.edigene.com

GTX104707_39694_20150410_IFA_2_19011506_540.jpg

GTX104707 ICC/IF Image

Stathmin 1 antibody detects Stathmin 1 protein at cytoplasm by immunofluorescent analysis.
Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Stathmin 1 protein stained by Stathmin 1 antibody (GTX104707) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Hoechst 33342 staining.

GTX104707_39694_WB_M_brain_19011506_153.jpg

GTX104707 WB Image

Stathmin 1 antibody detects STMN1 protein by Western blot analysis.
A. 50 µg mouse brain lysate/extract
12 % SDS-PAGE
Stathmin 1 antibody (GTX104707) dilution: 1:10000

GTX104707_39694_20170929_WB_M_R_19011506_344.jpg

GTX104707 WB Image

Various tissue extracts (50 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with Stathmin 1 antibody (GTX104707) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX104707_39694_20170807_IHC-Fr_M_19011506_834.jpg

GTX104707 IHC-Fr Image

Stathmin 1 antibody detects Stathmin 1 protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: Stathmin 1 protein stained by Stathmin 1 antibody (GTX104707) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

GTX104707_39694_WB_R_19011506_481.jpg

GTX104707 WB Image

Stathmin 1 antibody detects STMN1 protein by Western blot analysis.
A. 30 µg PC-12 whole cell lysate/extract
B. 30 µg Rat2 whole cell lysate/extract
12 % SDS-PAGE
Stathmin 1 antibody (GTX104707) dilution: 1:2000

GTX104707_39694_20150410_IFA_19011506_246.jpg

GTX104707 ICC/IF Image

Stathmin 1 antibody detects Stathmin 1 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Stathmin 1 protein stained by Stathmin 1 antibody (GTX104707) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] (GTX11304) diluted at 1:10000.
Blue: Hoechst 33342 staining.

GTX104707_39694_IHC_19011506_961.jpg

GTX104707 IHC-P Image

Stathmin 1 antibody detects Stathmin 1 protein at cytosol on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
Stathmin 1 antibody (GTX104707) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX104707_WB_19011506_439.jpg

GTX104707 WB Image

Sample(30 μg of whole cell lysate)
A:293T
B:A431(GTX27909)
C:H1299
12% SDS PAGE
GTX104707 diluted at 1:3000

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169