APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
H1299 , HeLa , mouse brain
Predict Reactivity
Rat, Bovine, Chicken, Pig, Xenopus laevis(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris-Glycine (pH7), 10% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.52 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human Stathmin 1. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2038052
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
stathmin 1 , C1orf215 , LAP18 , Lag , OP18 , PP17 , PP19 , PR22 , SMN
Cellular Localization
Membrane
Background
This gene belongs to the stathmin family of genes. It encodes a ubiquitous cytosolic phosphoprotein proposed to function as an intracellular relay integrating regulatory signals of the cellular environment. The encoded protein is involved in the regulation of the microtubule filament system by destabilizing microtubules. It prevents assembly and promotes disassembly of microtubules. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
Database
Research Area
DATA IMAGES
|
GTX113341 WB Image
Stathmin 1 antibody detects STMN1 protein by Western blot analysis.
A. 50 μg mouse brain lysate/extract
12 % SDS-PAGE
Stathmin 1 antibody (GTX113341) dilution: 1:500
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GTX113341 WB Image
Sample (30 ug of whole cell lysate)
A: H1299
B: Hela
12% SDS PAGE
GTX113341 diluted at 1:1000
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GTX113341 IHC-P Image
Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using Stathmin 1(GTX113341) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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REFERENCE
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