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Stathmin 1 (phospho Ser25) antibody

Anti-Stathmin 1 (phospho Ser25) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55035
Anti-Stathmin 1 (phospho Ser25) antibody used in Western Blot (WB). GTX55035
Anti-Stathmin 1 (phospho Ser25) antibody used in IHC (Paraffin sections) (IHC-P). GTX55035

Cat No. GTX55035

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($339)
See all Stathmin 1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:1000
ICC/IF 1:100 - 1:500
IHC-P 1:100 - 1:200
Not tested in other applications.

Calculated MW

17 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.42% Potassium Phosphate pH7.3, 0.87% Sodium Chloride, 30% Glycerol

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of Stathmin 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

stathmin 1 , C1orf215 , LAP18 , Lag , OP18 , PP17 , PP19 , PR22 , SMN

Cellular Localization

Cytoplasm, cytoskeleton

Background

This gene belongs to the stathmin family of genes. It encodes a ubiquitous cytosolic phosphoprotein proposed to function as an intracellular relay integrating regulatory signals of the cellular environment. The encoded protein is involved in the regulation of the microtubule filament system by destabilizing microtubules. It prevents assembly and promotes disassembly of microtubules. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2009]

Database

Research Area

DATA IMAGES

Anti-Stathmin 1 (phospho Ser25) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55035

GTX55035 ICC/IF Image

ICC/IF analysis of formalin-fixed HeLa cells using GTX55035 Stathmin 1 (phospho Ser25) antibody.
Red : Primary antibody
Blue : DAPI
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes

Anti-Stathmin 1 (phospho Ser25) antibody used in Western Blot (WB). GTX55035

GTX55035 WB Image

WB analysis of calyculin A-treated HeLa (A), Jurkat (B) whole cell lysates using GTX55035 Stathmin 1 (phospho Ser25) antibody.

Anti-Stathmin 1 (phospho Ser25) antibody used in IHC (Paraffin sections) (IHC-P). GTX55035

GTX55035 IHC-P Image

IHC-P analysis of formalin fixed human lung cancer tissue section using GTX55035 Stathmin 1 (phospho Ser25) antibody.
Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 339