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Staufen antibody

KO/KD Orthogonal
Anti-Staufen antibody used in Western Blot (WB). GTX106566
Anti-Staufen antibody used in Immunoprecipitation (IP). GTX106566
Anti-Staufen antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106566
Anti-Staufen antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106566
Anti-Staufen antibody used in Western Blot (WB). GTX106566

Cat. No. GTX106566

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IP

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all Staufen products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IP 1:500-1:3000
Not tested in other applications.

Calculated MW

63 kDa. ( Note )

Positive Control

Neuro2A

Product Note

Knockdown/Knockout validation was supported by references (PMID:26990993)

Predict Reactivity

Rat, Zebrafish, Chicken, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.93 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human Staufen. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1952044

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

staufen double-stranded RNA binding protein 1 , PPP1R150 , STAU

Cellular Localization

Rough endoplasmic reticulum

Background

Staufen is a member of the family of double-stranded RNA (dsRNA)-binding proteins involved in the transport and/or localization of mRNAs to different subcellular compartments and/or organelles. These proteins are characterized by the presence of multiple dsRNA-binding domains which are required to bind RNAs having double-stranded secondary structures. The human homologue of staufen encoded by STAU, in addition contains a microtubule- binding domain similar to that of microtubule-associated protein 1B, and binds tubulin. The STAU gene product has been shown to be present in the cytoplasm in association with the rough endoplasmic reticulum (RER), implicating this protein in the transport of mRNA via the microtubule network to the RER, the site of translation. Five transcript variants resulting from alternative splicing of STAU gene and encoding three isoforms have been described. Three of these variants encode the same isoform, however, differ in their 5'UTR. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Staufen antibody used in Western Blot (WB). GTX106566

GTX106566 WB Image

Sample (30 μg of whole cell lysate)
A: Neuro2A
GTX106566 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Staufen antibody used in Immunoprecipitation (IP). GTX106566

GTX106566 IP Image

Sample ( μg of whole cell lysate)
A: 293 (input)
B: control rabbit IgG-IP
C: Stau1-IP (GTX106566)
D: Post-IP lysate from control rabbit IgG-IP
E:Post-IP lysate from Stau1-IP
7.5% SDS PAGE
GTX106566 diluted at 1:3000
Image courtesy of Dr. Samantha Whitman (Laboratory of Dr. Donna Zhang, U of Arizona Pharmacology & Toxicology Department).
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Staufen antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106566

GTX106566 ICC/IF Image

Orthogonal

Staufen antibody detects Staufen protein by immunofluorescent analysis.
Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Staufen stained by Staufen antibody (GTX106566) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Staufen antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106566

GTX106566 ICC/IF Image

Staufen antibody detects Staufen protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Staufen stained by Staufen antibody (GTX106566) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Staufen antibody used in Western Blot (WB). GTX106566

GTX106566 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Staufen antibody (GTX106566) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Park MK et al. Cell Metab 2021; 33 (12) : 2380-2397.e9 NEAT1 is essential for metabolic changes that promote breast cancer growth and metastasis.
Application : WB
Reactivity : Human, Mouse
Sundararaman B et al. Mol Cell 2016; 61 (6) : 903-13 Resources for the Comprehensive Discovery of Functional RNA Elements.
Application : WB, IP
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169