Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Staufen antibody (GTX106566) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Staufen antibody detects Staufen protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Staufen stained by Staufen antibody (GTX106566) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Staufen antibody detects Staufen protein by immunofluorescent analysis.
Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Staufen stained by Staufen antibody (GTX106566) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Sample ( μg of whole cell lysate)
A: 293 (input)
B: control rabbit IgG-IP
C: Stau1-IP (GTX106566)
D: Post-IP lysate from control rabbit IgG-IP
E:Post-IP lysate from Stau1-IP
7.5% SDS PAGE
GTX106566 diluted at 1:3000
Image courtesy of Dr. Samantha Whitman (Laboratory of Dr. Donna Zhang, U of Arizona Pharmacology & Toxicology Department).
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Sample (30 μg of whole cell lysate)
A: Neuro2A
GTX106566 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.