GeneTex
United States (US)

Staufen antibody

KO/KD
GTX106566_40030_20181004_ICC_IF_R_19011519_307.jpg
GTX106566_40030_CT_WB_M_19011506_339.jpg
GTX106566_WB_19011506_626.jpg
GTX106566_40030_CT_WB_19011506_189.jpg

Cat No. GTX106566

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IP
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IP 1:500-1:3000
Not tested in other applications.

Calculated MW

63 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HepG2 , Molt-4 , Raji , Neuro2A , 293 (input) , Stau1-IP (GTX106566) , Post-IP lysate from control rabbit IgG-IP , Post-IP lysate from Stau1-IP

Specificity/Sensitivity

Knockdown/Knockout validation was supported by references (PMID:26990993)

Predict Reactivity

Rat, Zebrafish, Chicken, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.73mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human Staufen. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

FLJ25010 antibody, STAU antibody, STAU1 antibody, double-stranded RNA-binding protein Staufen homolog 1 antibody, "staufen, RNA binding protein, homolog 1 (Drosophila) antibody"

Cellular Localization

Rough endoplasmic reticulum

Background

Staufen is a member of the family of double-stranded RNA (dsRNA)-binding proteins involved in the transport and/or localization of mRNAs to different subcellular compartments and/or organelles. These proteins are characterized by the presence of multiple dsRNA-binding domains which are required to bind RNAs having double-stranded secondary structures. The human homologue of staufen encoded by STAU, in addition contains a microtubule- binding domain similar to that of microtubule-associated protein 1B, and binds tubulin. The STAU gene product has been shown to be present in the cytoplasm in association with the rough endoplasmic reticulum (RER), implicating this protein in the transport of mRNA via the microtubule network to the RER, the site of translation. Five transcript variants resulting from alternative splicing of STAU gene and encoding three isoforms have been described. Three of these variants encode the same isoform, however, differ in their 5'UTR. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX106566_40030_20181004_ICC_IF_R_19011519_307.jpg

GTX106566 ICC/IF Image

Staufen antibody detects Staufen protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Staufen stained by Staufen antibody (GTX106566) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GTX106566_40030_CT_WB_M_19011506_339.jpg

GTX106566 WB Image

Sample (30 µg of whole cell lysate)
A: Neuro2A
GTX106566 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX106566_WB_19011506_626.jpg

GTX106566 WB Image

Sample (30 µg of whole cell lysate)
A: HepG2 (GTX27900)
7.5% SDS PAGE
GTX106566 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX106566_40030_CT_WB_19011506_189.jpg

GTX106566 IP Image

Sample ( µg of whole cell lysate)
A: 293 (input)
B: control rabbit IgG-IP
C: Stau1-IP (GTX106566)
D: Post-IP lysate from control rabbit IgG-IP
E:Post-IP lysate from Stau1-IP
7.5% SDS PAGE
GTX106566 diluted at 1:3000
Image courtesy of Dr. Samantha Whitman (Laboratory of Dr. Donna Zhang, U of Arizona Pharmacology & Toxicology Department).
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169