Human tissue extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Steroid sulfatase antibody [N1C3] (GTX105498) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

HeLa whole cell and membrane extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Steroid sulfatase antibody [N1C3] (GTX105498) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded SW480 xenograft , using Steroid sulfatase (GTX105498) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min