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Steroid sulfatase antibody [N1C3]

Orthogonal
Anti-Steroid sulfatase antibody [N1C3] used in Western Blot (WB). GTX105498
Anti-Steroid sulfatase antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX105498
Anti-Steroid sulfatase antibody [N1C3] used in Western Blot (WB). GTX105498

Cat. No. GTX105498

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IHC

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all Steroid sulfatase products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IHC Assay dependent
Not tested in other applications.

Calculated MW

65 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.43 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Steroid sulfatase. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1952084

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

steroid sulfatase , ARSC , ARSC1 , ASC , ES , SSDD , XLI

Cellular Localization

Endoplasmic reticulum membrane; Multi-pass membrane protein

Background

The protein encoded by this gene catalyzes the conversion of sulfated steroid precursors to estrogens during pregnancy. The encoded protein is found in the endoplasmic reticulum, where it acts as a homodimer. Mutations in this gene are known to cause X-linked ichthyosis (XLI). [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Steroid sulfatase antibody [N1C3] used in Western Blot (WB). GTX105498

GTX105498 WB Image

Human tissue extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Steroid sulfatase antibody [N1C3] (GTX105498) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Steroid sulfatase antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX105498

GTX105498 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SW480 xenograft , using Steroid sulfatase (GTX105498) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Steroid sulfatase antibody [N1C3] used in Western Blot (WB). GTX105498

GTX105498 WB Image

Orthogonal

HeLa whole cell and membrane extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Steroid sulfatase antibody [N1C3] (GTX105498) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Calvillo-Robledo A et al. J Ovarian Res 2021; 14 (1) : 98 Simultaneous expression of steroid sulfatase and androgen receptor reduced overall survival of patients with epithelial ovarian tumors.
Application : IHC-P
Reactivity : Human
Mungenast F et al. Oncol Lett 2017; (Epub) Clinical significance of the estrogen-modifying enzymes steroid sulfatase and estrogen sulfotransferase in epithelial ovarian cancer.
Application : IHC-P
Reactivity : Human
Secky L et al. J Drug Deliv 2013; 2013 : 957605 The sulfatase pathway for estrogen formation: targets for the treatment and diagnosis of hormone-associated tumors.
Application : IHC
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169