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Steroid sulfatase antibody [N1C3]

Anti-Steroid sulfatase antibody [N1C3] used in Western Blot (WB). GTX105498
Anti-Steroid sulfatase antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX105498

Cat No. GTX105498

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, IHC
Reactivity Human
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IHC Assay dependent
Not tested in other applications.

Calculated MW

65 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.43 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Steroid sulfatase. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

steroid sulfatase , ARSC , ARSC1 , ASC , ES , SSDD , XLI

Cellular Localization

Endoplasmic reticulum membrane; Multi-pass membrane protein

Background

The protein encoded by this gene catalyzes the conversion of sulfated steroid precursors to estrogens during pregnancy. The encoded protein is found in the endoplasmic reticulum, where it acts as a homodimer. Mutations in this gene are known to cause X-linked ichthyosis (XLI). [provided by RefSeq]

Database

DATA IMAGES
Anti-Steroid sulfatase antibody [N1C3] used in Western Blot (WB). GTX105498

GTX105498 WB Image

HeLa whole cell and membrane extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Steroid sulfatase antibody [N1C3] (GTX105498) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Steroid sulfatase antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX105498

GTX105498 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SW480 xenograft , using Steroid sulfatase (GTX105498) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
REVIEW
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169