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Strep epitope Tag antibody [GT517]

Anti-Strep epitope Tag antibody [GT517] used in Western Blot (WB). GTX628899
Anti-Strep epitope Tag antibody [GT517] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628899

Cat. No. GTX628899

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT517

Isotype

 IgG1

Application

 WB, ICC/IF

Reactivity

 Species independent
Package
100 μl ($399),
25 μl ($169)
See all Strep epitope Tag products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Immunogen Amino acid sequence - ASWSHPQFEKGA

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888075

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Background

Strep epitope tag is a peptide sequence with high specificity and affinity toward Streptavidin.

Research Area

DATA IMAGES

Anti-Strep epitope Tag antibody [GT517] used in Western Blot (WB). GTX628899

GTX628899 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Strep epitope Tag antibody [GT517] (GTX628899) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Strep epitope Tag antibody [GT517] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628899

GTX628899 ICC/IF Image

Strep epitope Tag antibody [GT517] detects Strep-recombinant protein by immunofluorescent analysis. Sample: Strep/GFP-tag transfected HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Red: Strep-recombinant protein stained by Strep epitope Tag antibody [GT517] (GTX628899) diluted at 1:500. Green: GFP is expressed in the tranfected cell. Blue: Hoechst 33342 staining.

REFERENCE

Application Reference

Landeras-Bueno S et al. Cell Rep 2021; 35 (2) : 108986 Cellular mRNA triggers structural transformation of Ebola virus matrix protein VP40 to its essential regulatory form.
Application : WB
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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169