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Strep epitope Tag antibody [GT661]

Anti-Strep epitope Tag antibody [GT661] used in Western Blot (WB). GTX628900
Anti-Strep epitope Tag antibody [GT661] used in Western Blot (WB). GTX628900
Anti-Strep epitope Tag antibody [GT661] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628900
Anti-Strep epitope Tag antibody [GT661] used in Immunoprecipitation (IP). GTX628900

Cat No. GTX628900

Host

Mouse

Clonality

Monoclonal

Clone Name

GT661

Isotype

IgG2a

Application

WB, ICC/IF, IP
Package
100 μl ($379),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Positive Control

strep-tagged protein expressing 293T cells

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Immunogen Amino acid sequence - ASWSHPQFEKGA

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888076

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Background

Strep epitope tag is a peptide sequence with high specificity and affinity toward Streptavidin.

Research Area

DATA IMAGES

Anti-Strep epitope Tag antibody [GT661] used in Western Blot (WB). GTX628900

GTX628900 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Strep epitope Tag antibody [GT661] (GTX628900) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Strep epitope Tag antibody [GT661] used in Western Blot (WB). GTX628900

GTX628900 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Strep epitope Tag antibody [GT661] (GTX628900) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Strep epitope Tag antibody [GT661] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628900

GTX628900 ICC/IF Image

Strep epitope Tag antibody [GT661] detects Strep-recombinant protein by immunofluorescent analysis. Sample: Strep/GFP-tag transfected HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Red: Strep-recombinant protein stained by Strep epitope Tag antibody [GT661] (GTX628899) diluted at 1:500. Green: GFP is expressed in the tranfected cell. Blue: Hoechst 33342 staining.

Anti-Strep epitope Tag antibody [GT661] used in Immunoprecipitation (IP). GTX628900

GTX628900 IP Image

Strep epitope Tag antibody [GT661] immunoprecipitates strep tagged protein in IP experiments. IP Sample: 293T whole cell lysate/extract A : 20 μg whole cell lysate/extract of strep tagged protein expressing 293T cells B : Control with 2.5 μg of pre-immune mouse IgG C : Immunoprecipitation of strep tagged protein by 2.5 μg of Strep epitope Tag antibody [GT661] (GTX628900) 10% SDS-PAGE The immunoprecipitated strep tagged protein was detected by Strep epitope Tag antibody [GT661] (GTX628900) diluted at 1 : 3000. EasyBlot anti-mouse IgG (HRP) (GTX221667-01) was used as a secondary reagent.

REFERENCE

REVIEW

Strep epitope Tag antibody [GT661] Cat No. GTX628900

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 30-Apr-2019
Score :
Application Tested : WB
Sample : Strep-tagged protein expressing eHAP cells
Amount used : 10 μg
Blocking : 1% skim milk in PBS, 25°C, 2Hr
Primary Antibody : 1:1000, 4°C, 14Hr
SDS
PBS.pdf
Glycerol.pdf
Package List Price ($)
$ 379
$ 169