GeneTex
  • Country / Location Selection

United States (US)

Strep epitope Tag antibody [GT661]

Anti-Strep epitope Tag antibody [GT661] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628900
Anti-Strep epitope Tag antibody [GT661] used in Immunoprecipitation (IP). GTX628900
Anti-Strep epitope Tag antibody [GT661] used in Western Blot (WB). GTX628900

Cat No. GTX628900

Host

Mouse

Clonality

Monoclonal

Clone Name

GT661

Isotype

IgG2a

Application

WB, ICC/IF, IP
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Positive Control

strep-tagged protein expressing 293T cells

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7)

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Immunogen Amino acid sequence - ASWSHPQFEKGA

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Background

Strep epitope tag is a peptide sequence with high specificity and affinity toward Streptavidin.

Research Area

DATA IMAGES

Anti-Strep epitope Tag antibody [GT661] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628900

GTX628900 ICC/IF Image

Strep epitope Tag antibody [GT661] detects Strep-recombinant protein by immunofluorescent analysis. Sample: Strep/GFP-tag transfected HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Red: Strep-recombinant protein stained by Strep epitope Tag antibody [GT661] (GTX628899) diluted at 1:500. Green: GFP is expressed in the tranfected cell. Blue: Hoechst 33342 staining.

Anti-Strep epitope Tag antibody [GT661] used in Immunoprecipitation (IP). GTX628900

GTX628900 IP Image

Strep epitope Tag antibody [GT661] immunoprecipitates strep tagged protein in IP experiments. IP Sample: 293T whole cell lysate/extract A : 20 μg whole cell lysate/extract of strep tagged protein expressing 293T cells B : Control with 2.5 μg of pre-immune mouse IgG C : Immunoprecipitation of strep tagged protein by 2.5 μg of Strep epitope Tag antibody [GT661] (GTX628900) 10% SDS-PAGE The immunoprecipitated strep tagged protein was detected by Strep epitope Tag antibody [GT661] (GTX628900) diluted at 1 : 3000. EasyBlot anti-mouse IgG (HRP) (GTX221667-01) was used as a secondary reagent.

Anti-Strep epitope Tag antibody [GT661] used in Western Blot (WB). GTX628900

GTX628900 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Strep epitope Tag antibody [GT661] (GTX628900) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

REFERENCE

REVIEW

Strep epitope Tag antibody [GT661] Cat No. GTX628900

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 30-Apr-2019
Score :
Application Tested : WB
Sample : Strep-tagged protein expressing eHAP cells
Amount used : 10 μg
Blocking : 1% skim milk in PBS, 25°C, 2Hr
Primary Antibody : 1:1000, 4°C, 14Hr
SDS
PBS.pdf
Glycerol.pdf
Package List Price ($)
$ 329
$ 169