Strep epitope Tag antibody [GT661]

Name: Strep epitope Tag antibody [GT661]
Brand: GeneTex
SKU: GTX628900
Price: 399.00 USD
Rating: 5 (1 reviews)

Anti-Strep epitope Tag antibody [GT661] used in Immunoprecipitation (IP). GTX628900

Anti-Strep epitope Tag antibody [GT661] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628900

Anti-Strep epitope Tag antibody [GT661] used in Western Blot (WB). GTX628900

Cat. No. GTX628900

Reference 1

Host	Mouse
Clonality	Monoclonal
Clone Name	GT661
Isotype	IgG2a
Application	WB, ICC/IF, IP
Reactivity	Species independent
Package	100 μl ($399), 25 μl ($169)

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View product citations for antibody GTX628900 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:1000-1:10000
ICC/IF	1:100-1:1000
IP	1:100-1:500

Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Immunogen Amino acid sequence - ASWSHPQFEKGA

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888076

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Background

Strep epitope tag is a peptide sequence with high specificity and affinity toward Streptavidin.

Research Area

DATA IMAGES

GTX628900 IP Image

Strep epitope Tag antibody [GT661] immunoprecipitates strep tagged protein in IP experiments. IP Sample: 293T whole cell lysate/extract A : 20 μg whole cell lysate/extract of strep tagged protein expressing 293T cells B : Control with 2.5 μg of pre-immune mouse IgG C : Immunoprecipitation of strep tagged protein by 2.5 μg of Strep epitope Tag antibody [GT661] (GTX628900) 10% SDS-PAGE The immunoprecipitated strep tagged protein was detected by Strep epitope Tag antibody [GT661] (GTX628900) diluted at 1 : 3000. EasyBlot anti-mouse IgG (HRP) (GTX221667-01) was used as a secondary reagent.

GTX628900 ICC/IF Image

Strep epitope Tag antibody [GT661] detects Strep-recombinant protein by immunofluorescent analysis. Sample: Strep/GFP-tag transfected HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Red: Strep-recombinant protein stained by Strep epitope Tag antibody [GT661] (GTX628899) diluted at 1:500. Green: GFP is expressed in the tranfected cell. Blue: Hoechst 33342 staining.

GTX628900 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Strep epitope Tag antibody [GT661] (GTX628900) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Hori N et al. J Biol Chem 2020; 295 (26) : 8798-8807 Vestigial-like family member 3 (VGLL3), a cofactor for TEAD transcription factors, promotes cancer cell proliferation by activating the Hippo pathway.

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