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Strep epitope Tag antibody [HL1251]

Recombinant antibody
Anti-Strep epitope Tag antibody [HL1251] used in Immunoprecipitation (IP). GTX636653
Anti-Strep epitope Tag antibody [HL1251] used in Western Blot (WB). GTX636653
Anti-Strep epitope Tag antibody [HL1251] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636653

Cat No. GTX636653

Host

Rabbit

Clonality

Monoclonal

Clone Name

HL1251

Isotype

IgG

Application

WB, ICC/IF, IP
Package
100 μl ($379),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IP Assay dependent
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

The immunogen used to generate this antibody corresponds to Strep epitope Tag

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2910030

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

DATA IMAGES

Anti-Strep epitope Tag antibody [HL1251] used in Immunoprecipitation (IP). GTX636653

GTX636653 IP Image

Immunoprecipitation of Strep epitope Tag protein from Strep-tag tagged protein transfected 293T whole cell extracts using 5 μg of Strep epitope Tag antibody [HL1251] (GTX636653).
Western blot analysis was performed using Strep epitope Tag antibody [HL1251] (GTX636653).
EasyBlot HRP-conjugated anti FALSE.

Anti-Strep epitope Tag antibody [HL1251] used in Western Blot (WB). GTX636653

GTX636653 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Strep epitope Tag antibody [HL1251] (GTX636653) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Strep epitope Tag antibody [HL1251] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636653

GTX636653 ICC/IF Image

Strep epitope Tag antibody [HL1251] detects Strep epitope Tag protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Strep epitope Tag stained by Strep epitope Tag antibody [HL1251] (GTX636653) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

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REVIEW

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Package List Price ($)
$ 379
$ 169
Custom Conjugations