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Strep epitope Tag antibody

Anti-Strep epitope Tag antibody used in Western Blot (WB). GTX128061
Anti-Strep epitope Tag antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128061

Cat. No. GTX128061

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Species independent
Package
100 μl ($399),
25 μl ($169)
See all Strep epitope Tag products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF Assay dependent
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.31 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Immunogen Amino acid sequence - ASWSHPQFEKGA

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885697

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Background

Strep epitope tag is a peptide sequence with high specificity and affinity toward Streptavidin.

Research Area

DATA IMAGES

Anti-Strep epitope Tag antibody used in Western Blot (WB). GTX128061

GTX128061 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Strep epitope Tag antibody (GTX128061) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Strep epitope Tag antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128061

GTX128061 ICC/IF Image

Strep epitope Tag antibody detects Strep epitope Tag protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Strep epitope Tag stained by Strep epitope Tag antibody (GTX128061) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10μm.

REFERENCE

Application Reference

Healy MD et al. Cell 2023; 186 (10) : 2219-2237.e29 Structure of the endosomal Commander complex linked to Ritscher-Schinzel syndrome.
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169