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Strep epitope Tag antibody

Anti-Strep epitope Tag antibody used in Western Blot (WB). GTX128061
Anti-Strep epitope Tag antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128061

Cat No. GTX128061

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF
Package
100 μl ($379),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF Assay dependent
Not tested in other applications.

Positive Control

strep-tagged protein expressing 293T cells

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.31 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Immunogen Amino acid sequence - ASWSHPQFEKGA

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885697

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Background

Strep epitope tag is a peptide sequence with high specificity and affinity toward Streptavidin.

Research Area

DATA IMAGES

Anti-Strep epitope Tag antibody used in Western Blot (WB). GTX128061

GTX128061 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Strep epitope Tag antibody (GTX128061) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Strep epitope Tag antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128061

GTX128061 ICC/IF Image

Strep epitope Tag antibody detects Strep epitope Tag protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Strep epitope Tag stained by Strep epitope Tag antibody (GTX128061) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10μm.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 379
$ 169