GeneTex
United States (US)

Streptavidin(AP)

Cat No. GTX30954

Application WB, ELISA, IHC
PRODUCT

Product Description

Alkaline Phosphatase conjugated Streptavidin
APPLICATION

Application Note

ELISA: 1:2,000-1:20,000 (50-500 ng/ml); IHC: 1:200-1:1,000 (1-5 μg/ml), WB: 1:1,000- 1:10,000 (0.1-1.0 μg/ml). The optimum dilution should be determined by the individual investigator.
PROPERTIES

Form

Liquid

Buffer

10 mM Tris-HCl, 200 mM NaCl, 50% glycerol, pH8.0 buffer containing 10 mg of BSA and 0.05% sodium azide.

Storage

2-8ºC in dark, DO NOT FREEZE.

Conjugation

Alkaline Phosphatase (AP)

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Background

Streptavidin is a 55 kDa (subunit MW 14 kDa) biotin-binding protein isolated from Streptomyces avidini. Streptavidin is superior to avidin, because it does not contain carbohydrate like avidin and has no net charge at neutral pH. Streptavidin~biotin system is routinely used in Immunohistochemistry (IHC). Extinction Coefficient 1% A280=32.0; Purity: > 99% pure by HPLC; Eletrophoretically homogenous, single band;Protein content: > 95%; Activity 14.8 units/ mg, one unit bunds 1.0 microgram of d-biotin; Protease activity: 0.000018 units/mg; Isoelectric point: 7.0 +/- determined by Isoelectric focusing. Alkaline Phosphatase (ALP) used in this conjugation is 140 KDa enzyme purified from calf intestine.
Package List Price ($)
$ 179