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Streptavidin (Cy3)

Cat No. GTX85902

Application

ICC/IF, IHC-P, FACS
Package
500 μg ($149)
PRODUCT

Product Description

Streptavidin is a 52,800 dalton tetrameric protein purified from the bacterium Streptomyces avidinii. It has an extraordinarily high affinity for biotin and is one of the strongest non-covalent interactions known in nature. It is used extensively in biotechnology as, in addition to the high affinity, biotin-binding is resistant to extremes of pH, temperature, organic solvents, denaturants (e.g. guanidinium chloride), detergents (e.g. SDS, Triton) and proteolytic enzymes. Streptavidin conjugated to enzymes or fluorescent molecules is useful for the detection of biotinylated antibodies or proteins in immunological assays. For the detection of biotinylated molecules, streptavidin is superior to egg white avidin (avidin has a lower binding affinity for biotin conjugates, despite avidin having the higher affinity for free, unconjugated biotin).

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ICC/IF Assay dependent
IHC-P Assay dependent
FACS Assay dependent
Not tested in other applications.

Specificity/Sensitivity

Streptavidin binds biotin with a very high affinity.

PROPERTIES

Form

Liquid

Buffer

0.01M Sodium Phsphate, 0.25M NaCl (pH7.6), and 15 mg/mL BSA as a stabilizer.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4º C. Protect from light.

Conjugation

Cyanine3 (Cy3)

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Cy3-conjugated Streptavidin, Cy3conjugated Streptavidin, StreptavidinCy3, SACy3, SA-Cy3, Streptavidin-Cy3, SA Cy3, Streptavidin Cy3, Cy3 conjugated Streptavidin

Background

Streptavidin is a 52,800 dalton tetrameric protein purified from the bacterium Streptomyces avidinii. It has an extraordinarily high affinity for biotin and is one of the strongest non-covalent interactions known in nature. It is used extensively in biotechnology as, in addition to the high affinity, biotin-binding is resistant to extremes of pH, temperature, organic solvents, denaturants (e.g. guanidinium chloride), detergents (e.g. SDS, Triton) and proteolytic enzymes.Streptavidin conjugated to enzymes or fluorescent molecules is useful for the detection of biotinylated antibodies or proteins in immunological assays. For the detection of biotinylated molecules, streptavidin is superior to egg white avidin (avidin has a lower binding affinity for biotin conjugates, despite avidin having the higher affinity for free, unconjugated biotin).

REFERENCE

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Package List Price ($)
$ 149