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Streptavidin (FITC)

Cat No. GTX30950

Application ICC/IF, FACS
Package
1 mg ($149)
PRODUCT

Product Description

Streptavidin is a 55 kDa (subunit MW 14 kDa) biotin-binding protein isolated from Streptomyces avidini. Streptavidin is superior to avidin, because it does not contain carbohydrate like avidin and has no net charge at neutral pH. Streptavidin~biotin system is routinely used in Immunohistochemistry (IHC). Extinction Coefficient 1% A280=32.0. Protein content: > 95%. One mg of Streptavidin is conjugated with FITC, unconjugated Streptavidin is removed. This conjugate of streptavidin is recommended for use with Biotin~conjugated antibodies. Avidin~Biotin conjugated reagents are stable, sensitive and give less background. Fluorophore: Fluorescein isothiocyanate A max=492nm; E=520nm; Fluorophore/Protein: A492nm/A280nm=~1
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ICC/IF Assay dependent
FACS Assay dependent
Not tested in other applications.
PROPERTIES

Form

Liquid

Buffer

10 mM phosphate, 150 mM NaCl (pH 7.4), BSA 10 mg/ml and 0.05% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4º C. Protect from light.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Conjugation

Fluorescein isothiocyanate (FITC)

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Background

Streptavidin is a 55 kDa (subunit MW 14 kDa) biotin-binding protein isolated from Streptomyces avidini. Streptavidin is superior to avidin, because it does not contain carbohydrate like avidin and has no net charge at neutral pH. Streptavidin~biotin system is routinely used in Immunohistochemistry (IHC). Extinction Coefficient 1% A280=32.0. Purity: > 99% pure by HPLC; Eletrophoretically homogenous, single band. Protein content: > 95%; Activity 14.8 units/ mg, one unit bunds 1.0 microgram of d-biotin; Protease activity: 0.000018 units/mg; Isoelectric point: 7.0 +/- determined by Isoelectric focusing. One mg of Streptavidin is conjugated with FITC, unconjugated Streptavidin is removed. This conjugate of streptavidin is recommended for use with Biotin~conjugated antibodies. Avidin~Biotin conjugated reagents are stable, sensitive and give less background. Fluorophore: Fluorescein isothiocyanate A max=492nm; E=520nm; Fluorophore/Protein: A492nm/A280nm=~1
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Package List Price ($)
$ 149