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Streptavidin (PE)

Cat No. GTX85910

Application ICC/IF, FACS
Package
500 μl ($149)
PRODUCT

Product Description

Streptavidin is a 55 kDa (subunit MW 14 kDa) biotin-binding protein isolated from Streptomyces avidini. Streptavidin is superior to avidin, because it does not contain carbohydrate like avidin and has no net charge at neutral pH. Streptavidin~biotin system is routinely used in Immunohistochemistry (IHC). Extinction Coefficient 1% A280=32.0. Protein content: > 95%; Phycoethyrin (R-PE) is a 240 kDa phycobilliprotein found in red, macrophytic algae (seaweed). R-PE can be used with Fluorescein (FITC) for double labeling in flow cytometry using argon laser, since both R-PE and FITC can be excited at 488 nm, and R-PE fluoresces at a longer wavelength (580 nm) than FITC (520 nm). Phycoethyrin: R-PE purified from Poryhyra tenera; A565/A280 >5.0; A 1% 565 nm=82; Amax = 490, 545 and 566 nm; E max = 580 nm.
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ICC/IF Assay dependent
FACS Assay dependent
Not tested in other applications.
PROPERTIES

Form

Liquid

Buffer

10 mM phosphate, 150 mM NaCl, 1% BSA and 0.05% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4º C.

Conjugation

Phycoerythrin (PE)

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Background

Streptavidin is a 55 kDa (subunit MW 14 kDa) biotin-binding protein isolated from Streptomyces avidini. Streptavidin is superior to avidin, because it does not contain carbohydrate like avidin and has no net charge at neutral pH. Streptavidin~biotin system is routinely used in Immunohistochemistry (IHC). Extinction Coefficient 1% A280=32.0. Purity: > 99% pure by HPLC; Eletrophoretically homogenous, single band. Protein content: > 95%; Activity 14.8 units/ mg, one unit bunds 1.0 microgram of d-biotin; Protease activity: 0.000018 units/mg; Isoelectric point: 7.0 +/- determined by Isoelectric focusing. Phycoethyrin (R-PE) is a 240 kDa phycobilliprotein found in red, macrophytic algae (seaweed). R-PE can be used with Fluorescein (FITC) for double labeling in flow cytometry using argon laser, since both R-PE and FITC can be excited at 488 nm, and R-PE fluoresces at a longer wavelength (580 nm) than FITC (520 nm). Phycoethyrin: R-PE purified from Poryhyra tenera; A565/A280 >5.0; A 1% 565 nm=82; Amax = 490, 545 and 566 nm; E max = 580 nm.
REFERENCE
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Package List Price ($)
$ 149