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Streptavidin (TxRd)

Cat. No. GTX85907

Reference 1

Application

 ICC/IF, FACS
Package
500 μl ($129)
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PRODUCT

Summary

Streptavidin is a 55 kDa (subunit MW 14 kDa) biotin-binding protein isolated from Streptomyces avidini. Streptavidin is superior to avidin, because it does not contain carbohydrate like avidin and has no net charge at neutral pH. Streptavidin~biotin system is routinely used in Immunohistochemistry (IHC). Extinction Coefficient 1% A280=32.0; Protein content: > 95%; Texas Red: A max. 596 nm; E max. 620 nm. Fluorochrome / protein Absorbance ratio: A596 nm/ A280 nm.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF Assay dependent
FACS Assay dependent
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

10mM Phosphate, 150mM NaCl, 1% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. Protect from light.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Conjugation

Texas Red (TxRd)

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Background

Streptavidin is a 55 kDa (subunit MW 14 kDa) biotin-binding protein isolated from Streptomyces avidini. Streptavidin is superior to avidin, because it does not contain carbohydrate like avidin and has no net charge at neutral pH. Streptavidin~biotin system is routinely used in Immunohistochemistry (IHC). Extinction Coefficient 1% A280=32.0; Purity: Streptavidin used is 99% pure by HPLC; Eletrophoretically homogenous, single band, Protein content: > 95%; Activity 14.8 units/ mg, one unit bunds 1.0 microgram of d-biotin; Protease activity: 0.000018 units/mg; Isoelectric point: 7.0 +/- determined by Isoelectric focusing. Texas Red: A max. 596 nm; E max. 620 nm. Fluorochrome / protein Absorbance ratio: A596 nm/ A280 nm.

Research Area

REFERENCE

Application Reference

De Toni L et al. Sci Rep 2019; 9 (1) : 19432 SHBG141-161 Domain-Peptide Stimulates GPRC6A-Mediated Response in Leydig and β-Langerhans cell lines.
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SDS
Sodium Azide.pdf
Package List Price ($)
$ 129