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Streptavidin antibody (HRP)

Anti-Streptavidin antibody (HRP) used in ELISA (ELISA). GTX132626-01
Anti-Streptavidin antibody (HRP) used in Western Blot (WB). GTX132626-01

Cat. No. GTX132626-01

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ELISA
Package
100 μl ($399),
25 μl ($169)
See all Streptavidin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ELISA 1:1000-1:10000
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

0.05M Tris, 0.15M NaCl, 1%BSA

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

The immunogen used to generate this antibody corresponds to Streptavidin.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Horseradish peroxidase(HRP)

RRID

AB_2886697

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Streptavidin antibody (HRP) antibody , Streptavidin antibody

Research Area

DATA IMAGES

Anti-Streptavidin antibody (HRP) used in ELISA (ELISA). GTX132626-01

GTX132626-01 ELISA Image

An ELISA plate is coated with 50 μL of Streptavidin recombinant protein at concentration ranged from 0.03 μg/mL to 9 μg/mL. The coated protein is detected with HRP-conjugated anti-Streptavidin antibody (GTX132626-01) at 0.5 μg/mL.

Anti-Streptavidin antibody (HRP) used in Western Blot (WB). GTX132626-01

GTX132626-01 WB Image

Streptavidin (0.5 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Streptavidin antibody (GTX132626-01) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Arakelian T et al. Vaccine 2022; (Epub) Pyroptosis-inducing active caspase-1 as a genetic adjuvant in anti-cancer DNA vaccination.
Application : WB
Jessica Daughtry et al. ChemRxiv 2019; (Epub) Clickable Galactose Analogs for Imaging Glycans in Developing Zebrafish.
Yoon HW et al. Mol Immunol 2019; 114 : 62-71 Optimal combination of beneficial mutations for improved ADCC effector function of aglycosylated antibodies.
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REVIEW

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SDS
Proclin.pdf
Package List Price ($)
$ 399
$ 169