Identification of NADase inhibitor in crude extracts by western blotting with anti NADase inhibitor antibody. The antibody was used at 1/3,000 dilution. 1. Cell lysate of S. dysgalactiae 2. Cell lysate of E. coli overexpressing His-tagged NADase NADase inhibitor. 3. Culture supernatant of S. dysgalactiae 4. Molecular size markers.