GeneTex
United States (US)

Streptococcus pneumoniae antibody [0461]

Cat No. GTX36637

Host Mouse
Clonality Monoclonal
Clone Name 0461
Isotype IgG3
Application ICC/IF, ELISA
APPLICATION

Application Note

Recommended Starting Dilutions:
For ICC/IF: Use at 1:10-1:50
For WB: Use at 1:10-1:50
For IHC: Use at 1:10-1:50
For ELISA: Use at 1:20-1:200
Not yet tested in other applications. Optimal dilutions should be determined experimentally by the researcher.

Clone 0431 immunoprecipitates purified group A carbohydrate from both hot HCl & nitrous acid extracts. Works well HRP & gold conjugated. This monoclonal functions as a capture antibody on nitrocellulose but not on polystyrene solid phases. Clone 0721 immunoprecipitates purified group B carbohydrate from both autoclaved and enzyme prepared extracts but not HCl extracts.

Specificity/Sensitivity

Reactive with the Strep. pneumoniae common C-polysaccharide (backbone epitope)
PROPERTIES

Form

Liquid

Buffer

0.01 M phosphate buffered saline, pH 7.2 and contains 0.1% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

100 μg/ml (Please refer to the vial label for the specific concentration.)

Purification

Purified by protein A chromatography or sequential differential precipitations

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Package List Price ($)
$ 319