GeneTex
United States (US)

Sumo2 antibody

GTX124456_39757_WB_Z_18121410_327.jpg

Cat No. GTX124456

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Zebrafish
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

11 kDa. ( Note )

Positive Control

adult zebrafish
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.56 mg/ml (Please refer to the vial label for the specific concentration.)

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

SUMO-2 antibody, small ubiquitin-related modifier 2 antibody, zgc:92241 antibody, sumo2 antibody, SMT3 suppressor of mif two 3 homolog 2 (S. cerevisiae) antibody

Cellular Localization

Nucleus

Background

Ubiquitin-like protein that can be covalently attached to proteins as a monomer or as a lysine-linked polymer. Covalent attachment via an isopeptide bond to its substrates requires prior activation by the E1 complex sae1-sae2 and linkage to the E2 enzyme ube2i, and can be promoted by an E3 ligase such as pias1-4. This post-translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Polymeric sumo2 chains are also susceptible to polyubiquitination which functions as a signal for proteasomal degradation of modified proteins.

Database

Research Area

DATA IMAGES
GTX124456_39757_WB_Z_18121410_327.jpg

GTX124456 WB Image

Sample (30 ug of whole cell lysate)
A: adult zebrafish
15% SDS PAGE
GTX124456 diluted at 1:500

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 389
$ 199