Identification of Sup35 protein in crude extracts of S. cerevisiae by western blotting with anti-Sup35 antibody 1. Endogenous expression in S. cerevisiae. 2. Overexpression of Sup35 protein by introduction of a plasmid carrying Sup35 gene into S. cerevisiae. Anti-Sup35 antibody was used at 1/2,000 dilution. Sup35 protein was identified about 77 kDa position (Molecular mass of Sup35 is 76.6 kDa)