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Suppressor of Fused antibody

Anti-Suppressor of Fused antibody used in Western Blot (WB). GTX03498
Anti-Suppressor of Fused antibody used in IHC (Paraffin sections) (IHC-P). GTX03498
Anti-Suppressor of Fused antibody used in Western Blot (WB). GTX03498

Cat. No. GTX03498

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($409)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000 - 1:3000
IHC-P 1:50-1:200
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Predict Reactivity

Bovine, Dog, Horse, Xenopus(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human SUFU(Accession Q9UMX1), corresponding to amino acid residues M1-L29.

Purification

Purified by antigen-affinity chromatography
From serum

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

SUFU negative regulator of hedgehog signaling , JBTS32 , PRO1280 , SUFUH , SUFUXL

Cellular Localization

Cytoplasm , Nucleus

Background

The Hedgehog signaling pathway plays an important role in early human development. The pathway is a signaling cascade that plays a role in pattern formation and cellular proliferation during development. This gene encodes a negative regulator of the hedgehog signaling pathway. Defects in this gene are a cause of medulloblastoma. Alternative splicing results in multiple transcript variants.[provided by RefSeq, May 2010]

Database

Research Area

DATA IMAGES

Anti-Suppressor of Fused antibody used in Western Blot (WB). GTX03498

GTX03498 WB Image

WB analysis of HUVEC lysate using GTX03498 Suppressor of Fused antibody.

Anti-Suppressor of Fused antibody used in IHC (Paraffin sections) (IHC-P). GTX03498

GTX03498 IHC-P Image

IHC-P analysis of mouse testis tissue using GTX03498 Suppressor of Fused antibody.
Antigen retireval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100

Anti-Suppressor of Fused antibody used in Western Blot (WB). GTX03498

GTX03498 WB Image

WB analysis of MDA-MB-231 lysate using GTX03498 Suppressor of Fused antibody. The lane on the left was treated with blocking peptide.

REFERENCE

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REVIEW

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Package List Price ($)
$ 409