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Survivin antibody [1H5]

Anti-Survivin antibody [1H5] used in IHC (Paraffin sections) (IHC-P). GTX60534
Anti-Survivin antibody [1H5] used in Western Blot (WB). GTX60534
Anti-Survivin antibody [1H5] used in Flow cytometry (FACS). GTX60534
Anti-Survivin antibody [1H5] used in ELISA (ELISA). GTX60534
Anti-Survivin antibody [1H5] used in IHC (Paraffin sections) (IHC-P). GTX60534
Anti-Survivin antibody [1H5] used in Western Blot (WB). GTX60534
Anti-Survivin antibody [1H5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60534

Cat. No. GTX60534

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 1H5

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, FACS, ELISA

Reactivity

 Human
Package
100 μg ($409)
See all Survivin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
ICC/IF 1/200 - 1/1000
IHC-P 1/200 - 1/1000
FACS 1/200 - 1/400
ELISA 1/10000
Not tested in other applications.

Calculated MW

16 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified recombinant fragment of human BIRC5 expressed in E. Coli.

Purification

Protein G Purified

Conjugation

Unconjugated

RRID

AB_2887976

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

baculoviral IAP repeat containing 5 , API4 , EPR-1

Cellular Localization

Cytoplasm,Nucleus

Background

This gene is a member of the inhibitor of apoptosis (IAP) gene family, which encode negative regulatory proteins that prevent apoptotic cell death. IAP family members usually contain multiple baculovirus IAP repeat (BIR) domains, but this gene encodes proteins with only a single BIR domain. The encoded proteins also lack a C-terminus RING finger domain. Gene expression is high during fetal development and in most tumors, yet low in adult tissues. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jun 2011]

Database

Research Area

DATA IMAGES

Anti-Survivin antibody [1H5] used in IHC (Paraffin sections) (IHC-P). GTX60534

GTX60534 IHC-P Image

IHC-P analysis of cervical cancer tissue using GTX60534 Survivin antibody [1H5].

Anti-Survivin antibody [1H5] used in Western Blot (WB). GTX60534

GTX60534 WB Image

WB analysis of human Survivin (AA: 1-142) recombinant protein using GTX60534 Survivin antibody [1H5].

Anti-Survivin antibody [1H5] used in Flow cytometry (FACS). GTX60534

GTX60534 FACS Image

FACS analysis of Jurkat cells using GTX60534 Survivin antibody [1H5].
Green : Survivin
Red : negative control

Anti-Survivin antibody [1H5] used in ELISA (ELISA). GTX60534

GTX60534 ELISA Image

ELISA analysis of antigen using GTX60534 Survivin antibody [1H5].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng

Anti-Survivin antibody [1H5] used in IHC (Paraffin sections) (IHC-P). GTX60534

GTX60534 IHC-P Image

IHC-P analysis of colon cancer tissue using GTX60534 Survivin antibody [1H5].

Anti-Survivin antibody [1H5] used in Western Blot (WB). GTX60534

GTX60534 WB Image

WB analysis of HEK293 (1) and Survivin(AA: 1-142)-hIgGFc transfected HEK293 (2) cell lysate using GTX60534 Survivin antibody [1H5].

Anti-Survivin antibody [1H5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60534

GTX60534 ICC/IF Image

ICC/IF analysis of MSCS cells using GTX60534 Survivin antibody [1H5].
Green : Survivin
Blue: DRAQ5 fluorescent DNA dye
Red: Actin filaments

REFERENCE

Application Reference

Wang DW et al. Eur. Rev. Med. Pharmacol. Sci. 2018; 22 : 1241-1248 MiR-16 inhibits pituitary adenoma cell proliferation via the suppression of ERK/MAPK signal pathway.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 409