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Survivin antibody [2D9]

Overexpression
Anti-Survivin antibody [2D9] used in Western Blot (WB). GTX84824
Anti-Survivin antibody [2D9] used in Western Blot (WB). GTX84824
Anti-Survivin antibody [2D9] used in IHC (Paraffin sections) (IHC-P). GTX84824
Anti-Survivin antibody [2D9] used in Flow cytometry (FACS). GTX84824
Anti-Survivin antibody [2D9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84824
Anti-Survivin antibody [2D9] used in IHC (Paraffin sections) (IHC-P). GTX84824

Cat. No. GTX84824

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 2D9

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, FACS

Reactivity

 Human
Package
100 μl ($519)
See all Survivin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:2000
ICC/IF 1:100
IHC-P Assay dependent
FACS 1:100
Not tested in other applications.

Calculated MW

16 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human BIRC5 (NP_001159) produced in HEK293T cell.

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

baculoviral IAP repeat containing 5 , API4 , EPR-1

Cellular Localization

Cytoplasm,Nucleus

Background

Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. The complex with RAN plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules. May play a role in neoplasia. May counteract a default induction of apoptosis in G2/M phase. Inhibitor of caspase-3 and caspase-7. Isoform 2 and isoform 3 do not appear to play vital roles in mitosis. Isoform 3 shows a marked reduction in its anti-apoptotic effects when compared with the displayed wild-type isoform.

Database

Research Area

DATA IMAGES

Anti-Survivin antibody [2D9] used in Western Blot (WB). GTX84824

GTX84824 WB Image

WB analysis of human tissues (Lane 1-Testis ; Lane 2-Uterus ; Lane 3-Breast ; Lane 4-Brain ; Lane 5-Liver ; Lane 6-Ovary ; Lane 7-Thyroid gland ; Lane 8-colon) using GTX84824 Survivin antibody [2D9].
Loading : 10 ug per lane
Dilution : 1:200

Anti-Survivin antibody [2D9] used in Western Blot (WB). GTX84824

GTX84824 WB Image

Overexpression

WB analysis of HEK293T cells transfected with Survivin plasmid (Right) or empty vector (Left) for 48 hrs using GTX84824 Survivin antibody [2D9].
Loading : 5 ug per lane

Anti-Survivin antibody [2D9] used in IHC (Paraffin sections) (IHC-P). GTX84824

GTX84824 IHC-P Image

IHC-P analysis of human kidney tissue using GTX84824 Survivin antibody [2D9].
Antigen retrieval : Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH9.0, 120ºC for 3min.
Dilution : 1:50

Anti-Survivin antibody [2D9] used in Flow cytometry (FACS). GTX84824

GTX84824 FACS Image

FACS analysis of HEK293T cells transfected with either Survivin plasmid(Red) or empty vector control plasmid(Blue) using GTX84824 Survivin antibody [2D9].

Anti-Survivin antibody [2D9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84824

GTX84824 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with Survivin plasmid using GTX84824 Survivin antibody [2D9].

Anti-Survivin antibody [2D9] used in IHC (Paraffin sections) (IHC-P). GTX84824

GTX84824 IHC-P Image

IHC-P analysis of human liver tissue using GTX84824 Survivin antibody [2D9].
Antigen retrieval : Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH9.0, 120ºC for 3min.
Dilution : 1:50

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519