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Survivin antibody [60.11]

Overexpression
Anti-Survivin antibody [60.11] used in Western Blot (WB). GTX30241
Anti-Survivin antibody [60.11] used in IHC (Paraffin sections) (IHC-P). GTX30241
Anti-Survivin antibody [60.11] used in Western Blot (WB). GTX30241
Anti-Survivin antibody [60.11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX30241
Anti-Survivin antibody [60.11] used in Western Blot (WB). GTX30241

Cat. No. GTX30241

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 60.11

Isotype

 IgG2a

Application

 WB, ICC/IF, IHC-P, IHC-Fr, IP, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all Survivin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF 1:100
IHC-P Assay dependent
IHC-Fr Assay dependent
IP 1:10 - 1:500
ELISA Assay dependent
Not tested in other applications.

Calculated MW

16 kDa. ( Note )

Product Note

This antibody is specific for the cytoplasmic form of survivin.

PROPERTIES

Form

Liquid

Buffer

Ascites diluted in PBS

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Full length recombinant human Survivin [UniProt# O15392]

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

baculoviral IAP repeat containing 5 , API4 , EPR-1

Cellular Localization

Cytoplasm,Nucleus

Background

This gene is a member of the inhibitor of apoptosis (IAP) gene family, which encode negative regulatory proteins that prevent apoptotic cell death. IAP family members usually contain multiple baculovirus IAP repeat (BIR) domains, but this gene encodes proteins with only a single BIR domain. The encoded proteins also lack a C-terminus RING finger domain. Gene expression is high during fetal development and in most tumors yet low in adult tissues. Antisense transcripts are involved in the regulation of this gene's expression. At least four transcript variants encoding distinct isoforms have been found for this gene, but the full-length natures of only three of them have been determined. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Survivin antibody [60.11] used in Western Blot (WB). GTX30241

GTX30241 WB Image

Overexpression

WB analysis of mock or overexpressing Survivin cell lysate using GTX30241 Survivin antibody [60.11].

Anti-Survivin antibody [60.11] used in IHC (Paraffin sections) (IHC-P). GTX30241

GTX30241 IHC-P Image

IHC-P analysis of ovary cancer tissue using GTX30241 Survivin antibody [60.11].

Anti-Survivin antibody [60.11] used in Western Blot (WB). GTX30241

GTX30241 WB Image

WB analysis of HeLa cell lysate (30μg) using GTX30241 Survivin antibody [60.11].
Loading : 30μg
Dilution : 1 μg/ml

Anti-Survivin antibody [60.11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX30241

GTX30241 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX30241 Survivin antibody [60.11].
Green : primary antibody
Blue : Hoechst 33258

Anti-Survivin antibody [60.11] used in Western Blot (WB). GTX30241

GTX30241 WB Image

WB analysis of rat aorta smooth muscle tissue lysate using GTX30241 Survivin antibody [60.11].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399