GeneTex
United States (US)

Survivin antibody [60.11]

Overexpression
GTX30241_1372_WB_18121410_617.jpg
GTX30241_1370_WB_18121410_592.jpg
GTX30241_1371_WB_18121410_973.jpg
GTX30241_542_IHC-P_18121410_216.jpg
GTX30241_198_ICC-IF_18121410_207.jpg

Cat No. GTX30241

Host Mouse
Clonality Monoclonal
Clone Name 60.11
Isotype IgG2a
Application WB, ICC/IF, IHC-P, IHC-Fr, IP, ELISA
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF 1:100
IHC-P Assay dependent
IHC-Fr Assay dependent
IP 1:10 - 1:500
ELISA Assay dependent
Not tested in other applications.

Calculated MW

16 kDa. ( Note )

Specificity/Sensitivity

This antibody is specific for the cytoplasmic form of survivin.
PROPERTIES

Form

Liquid

Buffer

Ascites diulted with PBS, 0.05% Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length recombinant human Survivin [UniProt# O15392]

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Baculoviral Iap Repeat Containing 5,Api4,Epr-1,Birc5

Cellular Localization

Cytoplasm,Nucleus,Centromere

Background

This gene is a member of the inhibitor of apoptosis (IAP) gene family, which encode negative regulatory proteins that prevent apoptotic cell death. IAP family members usually contain multiple baculovirus IAP repeat (BIR) domains, but this gene encodes proteins with only a single BIR domain. The encoded proteins also lack a C-terminus RING finger domain. Gene expression is high during fetal development and in most tumors yet low in adult tissues. Antisense transcripts are involved in the regulation of this gene's expression. At least four transcript variants encoding distinct isoforms have been found for this gene, but the full-length natures of only three of them have been determined. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX30241_1372_WB_18121410_617.jpg

GTX30241 WB Image

WB analysis of rat aorta smooth muscle tissue lysate using GTX30241 Survivin antibody [60.11].

GTX30241_1370_WB_18121410_592.jpg

GTX30241 WB Image

WB analysis of HeLa cell lysate (30μg) using GTX30241 Survivin antibody [60.11].
Loading : 30μg
Dilution : 1 μg/ml

GTX30241_1371_WB_18121410_973.jpg

GTX30241 WB Image

Overexpression

WB analysis of mock or overexpressing Survivin cell lysate using GTX30241 Survivin antibody [60.11].

GTX30241_542_IHC-P_18121410_216.jpg

GTX30241 IHC-P Image

IHC-P analysis of ovary cancer tissue using GTX30241 Survivin antibody [60.11].

GTX30241_198_ICC-IF_18121410_207.jpg

GTX30241 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX30241 Survivin antibody [60.11].
Green : primary antibody
Blue : Hoechst 33258

Package List Price ($)
$ 339