GeneTex
United States (US)

Survivin antibody

GTX100052_39443_IFA_19011506_397.jpg
GTX100052_39443_20150410_IFA_19011506_292.jpg
GTX100052_39443_WB_19011506_702.jpg
GTX100052_39443_20151120_IP_19011506_967.jpg
GTX100052_IHC_19011506_272.jpg

Cat No. GTX100052

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

16 kDa. ( Note )

Positive Control

293T , HeLa

Predict Reactivity

Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Survivin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

API4 antibody, EPR-1 antibody, BIRC5 antibody, survivin variant 3 alpha antibody, apoptosis inhibitor survivin antibody, baculoviral IAP repeat-containing protein 5 antibody, apoptosis inhibitor 4 antibody, baculoviral IAP repeat containing 5 antibody

Cellular Localization

Cytoplasm , Nucleus , Centromere

Background

Survivin, also called baculoviral inhibitor of apoptosis repeat-containing 5 or BIRC5, is a protein that, in humans, is encoded by the BIRC5 gene.This gene is a member of the inhibitor of apoptosis (IAP) gene family, which encode negative regulatory proteins that prevent apoptotic cell death. IAP family members usually contain multiple baculovirus IAP repeat (BIR) domains, but this gene encodes proteins with only a single BIR domain. The encoded proteins also lack a C-terminus RING finger domain. Gene expression is high during fetal development and in most tumors yet low in adult tissues. Antisense transcripts are involved in the regulation of this gene's expression. At least four transcript variants encoding distinct isoforms have been found for this gene, but the full-length natures of only three of them have been determined. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX100052_39443_IFA_19011506_397.jpg

GTX100052 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed U2OS, using Survivin(GTX100052) antibody (Geen) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

GTX100052_39443_20150410_IFA_19011506_292.jpg

GTX100052 ICC/IF Image

Survivin antibody detects Survivin protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Survivin protein stained by Survivin antibody (GTX100052) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Hoechst 33342 staining.

GTX100052_39443_WB_19011506_702.jpg

GTX100052 WB Image

Survivin antibody detects Survivin protein by western blot analysis.
A. 30 µg 293T whole cell lysate/extract
B. 30 µg HeLa whole cell lysate/extract
12% SDS-PAGE
Survivin antibody (GTX100052) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100052_39443_20151120_IP_19011506_967.jpg

GTX100052 IP Image

Immunoprecipitation of Survivin protein from 293T whole cell extracts using 5 μg of Survivin antibody (GTX100052).
Western blot analysis was performed using Survivin antibody (GTX100052).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX100052_IHC_19011506_272.jpg

GTX100052 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human gastric cancer, using survivin (GTX100052) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
REVIEW

Survivin antibody Cat No. GTX100052

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 08-Jan-2018
Score :
Application Tested : WB
Sample Species : Hu
Amount used : 25 μg
Blocking : 5% milk in TBST, 25°C, 1Hr
Primary Antibody : 1:500, 4°C, overnightHr
Lane Description : Lane 1: PANC1, Lane 2: AsPC1, Lane 3: BxPC3
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Document
Unmodified.pdf
Package List Price ($)
$ 319
$ 169