GeneTex
United States (US)

Survivin antibody

GTX100441_WB_M_19011506_811.jpg
GTX100441_WB_19011506_395.jpg
GTX100441_39631_IHC_2_19011506_728.jpg
GTX100441_39631_20151120_IP_19011506_571.jpg
GTX100441_39631_IHC_19011506_305.jpg
GTX100441_39631_IFA_19011506_528.jpg

Cat No. GTX100441

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

16 kDa. ( Note )

Positive Control

293T , NIH-3T3
PROPERTIES

Form

Liquid

Buffer

1XPBS, 40% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Survivin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Baculoviral Iap Repeat Containing 5,Api4,Epr-1,Birc5

Cellular Localization

Cytoplasm , Nucleus , Centromere

Background

Multitasking protein that has dual roles in promoting cell proliferation and preventing apoptosis. Component of a chromosome passage protein complex (CPC) which is essential for chromosome alignment and segregation during mitosis and cytokinesis. Acts as an important regulator of the localization of this complex; directs CPC movement to different locations from the inner centromere during prometaphase to midbody during cytokinesis and participates in the organization of the center spindle by associating with polymerized microtubules. The complex with RAN plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules. May counteract a default induction of apoptosis in G2/M phase. The acetylated form represses STAT3 transactivation of target gene promoters. May play a role in neoplasia. Inhibitor of CASP3 and CASP7. Isoform 2 and isoform 3 do not appear to play vital roles in mitosis. Isoform 3 shows a marked reduction in its anti-apoptotic effects when compared with the displayed wild-type isoform.

Database

Research Area

DATA IMAGES
GTX100441_WB_M_19011506_811.jpg

GTX100441 WB Image

Sample (30 µg of whole cell lysate)
A:NIH-3T3
15% SDS PAGE
GTX100441 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100441_WB_19011506_395.jpg

GTX100441 WB Image

Sample(30 µg of whole cell lysate)
A:293T
15% SDS PAGE
GTX100441 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100441_39631_IHC_2_19011506_728.jpg

GTX100441 IHC-P Image

Survivin antibody detects Survivin protein at cytosol on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human colon carcinoma.
Survivin antibody (GTX100441) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100441_39631_20151120_IP_19011506_571.jpg

GTX100441 IP Image

Immunoprecipitation of Survivin protein from 293T whole cell extracts using 5 μg of Survivin antibody (GTX100441).
Western blot analysis was performed using Survivin antibody (GTX100441).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX100441_39631_IHC_19011506_305.jpg

GTX100441 IHC-P Image

Survivin antibody detects Survivin protein at cytosol on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
Survivin antibody (GTX100441) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100441_39631_IFA_19011506_528.jpg

GTX100441 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed U2OS, using Survivin(GTX100441) antibody (Geen) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169