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Survivin antibody

Anti-Survivin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100441
Anti-Survivin antibody used in Immunoprecipitation (IP). GTX100441
Anti-Survivin antibody used in IHC (Paraffin sections) (IHC-P). GTX100441
Anti-Survivin antibody used in IHC (Paraffin sections) (IHC-P). GTX100441
Anti-Survivin antibody used in Western Blot (WB). GTX100441
Anti-Survivin antibody used in Western Blot (WB). GTX100441

Cat. No. GTX100441

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse
Package
100 μl ($419),
25 μl ($169)
See all Survivin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

16 kDa. ( Note )

Positive Control

293T , NIH-3T3

PROPERTIES

Form

Liquid

Buffer

PBS, 40% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Survivin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1241367

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

baculoviral IAP repeat containing 5 , API4 , EPR-1

Cellular Localization

Cytoplasm , Nucleus , Centromere

Background

Multitasking protein that has dual roles in promoting cell proliferation and preventing apoptosis. Component of a chromosome passage protein complex (CPC) which is essential for chromosome alignment and segregation during mitosis and cytokinesis. Acts as an important regulator of the localization of this complex; directs CPC movement to different locations from the inner centromere during prometaphase to midbody during cytokinesis and participates in the organization of the center spindle by associating with polymerized microtubules. The complex with RAN plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules. May counteract a default induction of apoptosis in G2/M phase. The acetylated form represses STAT3 transactivation of target gene promoters. May play a role in neoplasia. Inhibitor of CASP3 and CASP7. Isoform 2 and isoform 3 do not appear to play vital roles in mitosis. Isoform 3 shows a marked reduction in its anti-apoptotic effects when compared with the displayed wild-type isoform.

Database

Research Area

DATA IMAGES

Anti-Survivin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100441

GTX100441 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed U2OS, using Survivin(GTX100441) antibody (Geen) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Anti-Survivin antibody used in Immunoprecipitation (IP). GTX100441

GTX100441 IP Image

Immunoprecipitation of Survivin protein from 293T whole cell extracts using 5 μg of Survivin antibody (GTX100441).
Western blot analysis was performed using Survivin antibody (GTX100441).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-Survivin antibody used in IHC (Paraffin sections) (IHC-P). GTX100441

GTX100441 IHC-P Image

Survivin antibody detects Survivin protein at cytosol on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human colon carcinoma.
Survivin antibody (GTX100441) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Survivin antibody used in IHC (Paraffin sections) (IHC-P). GTX100441

GTX100441 IHC-P Image

Survivin antibody detects Survivin protein at cytosol on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
Survivin antibody (GTX100441) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Survivin antibody used in Western Blot (WB). GTX100441

GTX100441 WB Image

Sample(30 μg of whole cell lysate)
A:293T
15% SDS PAGE
GTX100441 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Survivin antibody used in Western Blot (WB). GTX100441

GTX100441 WB Image

Sample (30 μg of whole cell lysate)
A:NIH-3T3
15% SDS PAGE
GTX100441 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Wuputra K et al. Int J Mol Sci 2022; 23 (14) FOXM1-CD44 Signaling Is Critical for the Acquisition of Regorafenib Resistance in Human Liver Cancer Cells.
Magarin M et al. J Mol Cell Cardiol 2016; 97 : 43114 Embryonic cardiomyocytes can orchestrate various cell protective mechanisms to survive mitochondrial stress.
Application : IHC-P
Reactivity : Mouse
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 419
$ 169