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Syk antibody [GT351]

Anti-Syk antibody [GT351] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX633910
Anti-Syk antibody [GT351] used in Western Blot (WB). GTX633910

Cat. No. GTX633910

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT351

Isotype

 IgG1

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all Syk products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

72 kDa. ( Note )

Positive Control

Raji

Predict Reactivity

Mouse, Rat, Bovine, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2.83 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Syk. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.
From tissue culture supernatant

Conjugation

Unconjugated

RRID

AB_2888388

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

spleen associated tyrosine kinase , p72-Syk

Cellular Localization

Cell membrane,Cytoplasm, cytosol

Background

This gene encodes a member of the family of non-receptor type Tyr protein kinases. This protein is widely expressed in hematopoietic cells and is involved in coupling activated immunoreceptors to downstream signaling events that mediate diverse cellular responses, including proliferation, differentiation, and phagocytosis. It is thought to be a modulator of epithelial cell growth and a potential tumour suppressor in human breast carcinomas. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2010]

Database

Research Area

DATA IMAGES

Anti-Syk antibody [GT351] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX633910

GTX633910 ICC/IF Image

Syk antibody [GT351] detects Syk protein at cytoplasm and cell membrane by immunofluorescent analysis.
Sample: Jurkat cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Syk stained by Syk antibody [GT351] (GTX633910) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10 μm.

Anti-Syk antibody [GT351] used in Western Blot (WB). GTX633910

GTX633910 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Syk antibody [GT351] (GTX633910) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations