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Syk antibody, Internal

Anti-Syk antibody, Internal used in Western Blot (WB). GTX89525

Cat. No. GTX89525

Host

Goat

Clonality

Polyclonal

Isotype

IgG

Application

WB

Reactivity

Human
Package
100 μg ($399)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1-3μg/ml
Not tested in other applications.

Calculated MW

72 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX89525-PEP).

Product Note

This antibody is expected to recognise both reported isoforms (NP_003168.2; NP_001128524.1).

Predict Reactivity

Mouse, Rat, Bovine, Dog, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

TBS pH7.3, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-KYLQQNRHVKDKN, from the internal region of the protein sequence according to NP_003168.2; NP_001128524.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

spleen associated tyrosine kinase , p72-Syk

Cellular Localization

Cell membrane , Cytoplasm, cytosol

Background

This gene encodes a member of the family of non-receptor type Tyr protein kinases. This protein is widely expressed in hematopoietic cells and is involved in coupling activated immunoreceptors to downstream signaling events that mediate diverse cellular responses, including proliferation, differentiation, and phagocytosis. It is thought to be a modulator of epithelial cell growth and a potential tumour suppressor in human breast carcinomas. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2010]

Database

Research Area

DATA IMAGES

Anti-Syk antibody, Internal used in Western Blot (WB). GTX89525

GTX89525 WB Image

WB analysis of human spleen lysate using GTX89525 Syk antibody, Internal.
Dilution : 1μg/ml
Loading : 35μg protein in RIPA buffer

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399