GeneTex
United States (US)

Syk antibody [N1C1]

Anti-Syk antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX107459
Anti-Syk antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX107459
Anti-Syk antibody [N1C1] used in Western Blot (WB). GTX107459

Cat No. GTX107459

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

72 kDa. ( Note )

Positive Control

A431 , Raji

Predict Reactivity

Rat, Bovine, Chicken, Pig, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.02 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Syk. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Spleen Associated Tyrosine Kinase,P72-Syk,Syk

Background

This gene encodes a member of the family of non-receptor type Tyr protein kinases. This protein is widely expressed in hematopoietic cells and is involved in coupling activated immunoreceptors to downstream signaling events that mediate diverse cellular responses, including proliferation, differentiation, and phagocytosis. It is thought to be a modulator of epithelial cell growth and a potential tumour suppressor in human breast carcinomas. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-Syk antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX107459

GTX107459 IHC-P Image

Immunohistochemical analysis of paraffin-embedded H1299 xenograft, using SYK(GTX107459) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Syk antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX107459

GTX107459 IHC-P Image

SYK antibody [N1C1] detects SYK protein on mouse spleen by immunohistochemical analysis.
Sample: Paraffin-embedded mouse spleen.
SYK antibody [N1C1] (GTX107459) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Syk antibody [N1C1] used in Western Blot (WB). GTX107459

GTX107459 WB Image

Sample(30 ug whole cell lysate)
A:Raji (GTX27908)
7.5% SDS PAGE
GTX107459 diluted at 1:500

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169