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Syk antibody [N2C2], Internal

Anti-Syk antibody [N2C2], Internal used in Western Blot (WB). GTX100748
Anti-Syk antibody [N2C2], Internal used in Western Blot (WB). GTX100748
Anti-Syk antibody [N2C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100748
Anti-Syk antibody [N2C2], Internal used in Immunoprecipitation (IP). GTX100748
Anti-Syk antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX100748
Anti-Syk antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX100748
Anti-Syk antibody [N2C2], Internal used in Western Blot (WB). GTX100748
Anti-Syk antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX100748
Anti-Syk antibody [N2C2], Internal used in Western Blot (WB). GTX100748

Cat. No. GTX100748

Reference 4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Syk products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

72 kDa. ( Note )

Positive Control

mouse BMDM cells , mouse spleen , Raji , A431

Predict Reactivity

Bovine, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.32 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Syk. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1952121

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

spleen associated tyrosine kinase , p72-Syk

Background

This gene encodes a member of the family of non-receptor type Tyr protein kinases. This protein is widely expressed in hematopoietic cells and is involved in coupling activated immunoreceptors to downstream signaling events that mediate diverse cellular responses, including proliferation, differentiation, and phagocytosis. It is thought to be a modulator of epithelial cell growth and a potential tumour suppressor in human breast carcinomas. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Syk antibody [N2C2], Internal used in Western Blot (WB). GTX100748

GTX100748 WB Image

SYK antibody [N2C2], Internal detects SYK protein by western blot analysis.
A. 30 μg mouse BMDM (bone marrow-derived macrophage) cells
B. 30 μg mouse Syk null cells
10% SDS-PAGE
SYK antibody [N2C2], Internal (GTX100748) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Syk antibody [N2C2], Internal used in Western Blot (WB). GTX100748

GTX100748 WB Image

SYK antibody detects SYK protein by western blot analysis. Whole cell extracts (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with SYK antibody (GTX100748) at a dilution of 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Syk antibody [N2C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100748

GTX100748 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A431, using SYK(GTX100748) antibody at 1:200 dilution.

Anti-Syk antibody [N2C2], Internal used in Immunoprecipitation (IP). GTX100748

GTX100748 IP Image

Immunoprecipitation of SYK protein from A431 whole cell extracts using 5 μg of SYK antibody [N2C2] (GTX100748).
Western blot analysis was performed using SYK antibody [N2C2] (GTX100748).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-Syk antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX100748

GTX100748 IHC-P Image

SYK antibody [N2C2], Internal detects SYK protein at cytoplasm in human endometrium by immunohistochemical analysis.
Sample: Paraffin-embedded human endometrium.
SYK antibody [N2C2], Internal (GTX100748) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Syk antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX100748

GTX100748 IHC-P Image

SYK antibody [N2C2], Internal detects SYK protein at cytosol on rat spleen by immunohistochemical analysis.
Sample: Paraffin-embedded rat spleen.
SYK antibody [N2C2], Internal (GTX100748) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Syk antibody [N2C2], Internal used in Western Blot (WB). GTX100748

GTX100748 WB Image

SYK antibody detects SYK protein by western blot analysis. Mouse tissue extracts (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with SYK antibody (GTX100748) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Syk antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX100748

GTX100748 IHC-P Image

SYK antibody [N2C2], Internal detects SYK protein at cytoplasm in human cervical cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human cervical cancer.
SYK antibody [N2C2], Internal (GTX100748) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Syk antibody [N2C2], Internal used in Western Blot (WB). GTX100748

GTX100748 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Syk antibody [N2C2], Internal (GTX100748) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Tsukui D et al. iScience 2023; 26 (8) : 107293 GM-CSF receptor/SYK/JNK/FOXO1/CD11c signaling promotes atherosclerosis.
Jia Y et al. Cell Death Dis 2019; 10 (2) : 38 Tetrandrine enhances the ubiquitination and degradation of Syk through an AhR-c-src-c-Cbl pathway and consequently inhibits osteoclastogenesis and bone destruction in arthritis.
Application : WB
Reactivity : Mouse
Jia Y et al. FASEB J 2018; : fj201701148RR Tetrandrine attenuates the bone erosion in collagen-induced arthritis rats by inhibiting osteoclastogenesis via spleen tyrosine kinase.
Application : WB
Reactivity : Rat
Tomoyuki Mukai et al. J Bone Miner Res 2014; 29 (12) : 2618-2635 SH3BP2 Cherubism Mutation Potentiates TNF‐α–Induced Osteoclastogenesis via NFATc1 and TNF‐α–Mediated Inflammatory Bone Loss.
Application : WB
Reactivity : Mouse
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169