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Syk antibody [N2C2], Internal

GTX100748_40051_WB_M_19011506_106.jpg
GTX100748_41990_20150518_IHC-P_19011506_576.jpg
GTX100748_41906_20150119_WB_19011506_138.jpg
GTX100748_41990_20150518_IHC-P_2_19011506_560.jpg
GTX100748_40051_IFA_19011506_288.jpg
GTX100748_40051_20141024_IHC_R_19011506_375.jpg
GTX100748_41892_20150112_IP_19011506_109.jpg
GTX100748_41892_20141212_WB_M_spleen_19011506_442.jpg
GTX100748_41906_20150119_WB_2_19011506_388.jpg

Cat No. GTX100748

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

72 kDa. ( Note )

Positive Control

A431 , Raji , mouse BMDM , mouse spleen

Predict Reactivity

Bovine, Pig, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.32mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Syk. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

DKFZp313N1010 antibody, FLJ25043 antibody, FLJ37489 antibody, SYK antibody, tyrosine-protein kinase SYK antibody, spleen tyrosine kinase antibody

Background

This gene encodes a member of the family of non-receptor type Tyr protein kinases. This protein is widely expressed in hematopoietic cells and is involved in coupling activated immunoreceptors to downstream signaling events that mediate diverse cellular responses, including proliferation, differentiation, and phagocytosis. It is thought to be a modulator of epithelial cell growth and a potential tumour suppressor in human breast carcinomas. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX100748_40051_WB_M_19011506_106.jpg

GTX100748 WB Image

SYK antibody [N2C2], Internal detects SYK protein by western blot analysis.
A. 30 µg mouse BMDM (bone marrow-derived macrophage) cells
B. 30 µg mouse Syk null cells
10% SDS-PAGE
SYK antibody [N2C2], Internal (GTX100748) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100748_41990_20150518_IHC-P_19011506_576.jpg

GTX100748 IHC-P Image

SYK antibody [N2C2], Internal detects SYK protein at cytoplasm in human cervical cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human cervical cancer.
SYK antibody [N2C2], Internal (GTX100748) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX100748_41906_20150119_WB_19011506_138.jpg

GTX100748 WB Image

SYK antibody detects SYK protein by western blot analysis. Whole cell extracts (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with SYK antibody (GTX100748) at a dilution of 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100748_41990_20150518_IHC-P_2_19011506_560.jpg

GTX100748 IHC-P Image

SYK antibody [N2C2], Internal detects SYK protein at cytoplasm in human endometrium by immunohistochemical analysis.
Sample: Paraffin-embedded human endometrium.
SYK antibody [N2C2], Internal (GTX100748) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX100748_40051_IFA_19011506_288.jpg

GTX100748 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A431, using SYK(GTX100748) antibody at 1:200 dilution.

GTX100748_40051_20141024_IHC_R_19011506_375.jpg

GTX100748 IHC-P Image

SYK antibody [N2C2], Internal detects SYK protein at cytosol on rat spleen by immunohistochemical analysis.
Sample: Paraffin-embedded rat spleen.
SYK antibody [N2C2], Internal (GTX100748) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100748_41892_20150112_IP_19011506_109.jpg

GTX100748 IP Image

Immunoprecipitation of SYK protein from A431 whole cell extracts using 5 μg of SYK antibody [N2C2] (GTX100748).
Western blot analysis was performed using SYK antibody [N2C2] (GTX100748).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX100748_41892_20141212_WB_M_spleen_19011506_442.jpg

GTX100748 WB Image

SYK antibody detects SYK protein by western blot analysis. Mouse tissue extracts (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with SYK antibody (GTX100748) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100748_41906_20150119_WB_2_19011506_388.jpg

GTX100748 WB Image

SYK antibody detects SYK protein by western blot analysis. Whole cell extracts (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with SYK antibody (GTX100748) at a dilution of 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169