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Syk (phospho Tyr323) antibody

Anti-Syk (phospho Tyr323) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50355
Anti-Syk (phospho Tyr323) antibody used in IHC (Paraffin sections) (IHC-P). GTX50355
Anti-Syk (phospho Tyr323) antibody used in Western Blot (WB). GTX50355

Cat No. GTX50355

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human
Package
100 μl ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:1000
ICC/IF 1:100-1:200
IHC-P 1:50-1:100
Not tested in other applications.

Calculated MW

72 kDa. ( Note )

Predict Reactivity

Mouse, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH 7.4, 150mM NaCl, 0.02% sodium azide, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of tyrosine 323 (N-P-Y(p)-E- P) derived from human Syk.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

spleen associated tyrosine kinase , p72-Syk

Cellular Localization

Cell membrane,Cytoplasm, cytosol

Background

This gene encodes a member of the family of non-receptor type Tyr protein kinases. This protein is widely expressed in hematopoietic cells and is involved in coupling activated immunoreceptors to downstream signaling events that mediate diverse cellular responses, including proliferation, differentiation, and phagocytosis. It is thought to be a modulator of epithelial cell growth and a potential tumour suppressor in human breast carcinomas. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2010]

Database

Research Area

DATA IMAGES

Anti-Syk (phospho Tyr323) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50355

GTX50355 ICC/IF Image

ICC/IF analysis of methanol-fixed HeLa cells using GTX50355 Syk (phospho Tyr323) antibody.

Anti-Syk (phospho Tyr323) antibody used in IHC (Paraffin sections) (IHC-P). GTX50355

GTX50355 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX50355 Syk (phospho Tyr323) antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

Anti-Syk (phospho Tyr323) antibody used in Western Blot (WB). GTX50355

GTX50355 WB Image

WB analysis of extracts from JK cells untreated or treated with H2O2 for the indicated times using GTX50355 Syk (phospho Tyr323) antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 319