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Symplekin antibody, N-term

Anti-Symplekin antibody, N-term used in Western Blot (WB). GTX46952
Anti-Symplekin antibody, N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX46952

Cat. No. GTX46952

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
50 μg ($399)
See all Symplekin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.2-2.5 ug/ml
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

141 kDa. ( Note )

Positive Control

human fetal liver

PROPERTIES

Form

Liquid

Buffer

PBS, 2% Sucrose

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5-1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to a N-terminal region of Human Symplekin.

Purification

Affinity Purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

symplekin , SPK , SYM

Cellular Localization

Cytoplasm, cytoskeleton,Cell junction,nucleoplasm

Background

This gene encodes a nuclear protein that functions in the regulation of polyadenylation and promotes gene expression. The protein forms a high-molecular weight complex with components of the polyadenylation machinery. It is thought to serve as a scaffold for recruiting regulatory factors to the polyadenylation complex. It also participates in 3'-end maturation of histone mRNAs, which do not undergo polyadenylation. The protein also localizes to the cytoplasmic plaques of tight junctions in some cell types. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Symplekin antibody, N-term used in Western Blot (WB). GTX46952

GTX46952 WB Image

WB analysis of human fetal liver tissue using GTX46952 SYMPK antibody at 0.2-1μg/ml.

Anti-Symplekin antibody, N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX46952

GTX46952 ICC/IF Image

ICC/IF analysis of HT29 cells using GTX46952 SYMPK antibody at 1:50.
Green: SYMPK
Blue: DAPI

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399