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Synapsin I antibody

Anti-Synapsin I antibody used in Western Blot (WB). GTX82594
Anti-Synapsin I antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX82594

Cat. No. GTX82594

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-Fr, IP, IHC

Reactivity

 Human, Mouse, Rat
Package
10 μg ($499)
See all Synapsin I products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-Fr Assay dependent
IP Assay dependent
IHC Assay dependent
Not tested in other applications.

Calculated MW

75 kDa. ( Note )

PROPERTIES

Form

Liquid

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Bovine

Immunogen

Native protein purified from bovine brain

Purification

Affinity Purified

Conjugation

Unconjugated

RRID

AB_11173759

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Cellular Localization

Cell junction, synapse,Golgi apparatus

Database

Research Area

DATA IMAGES

Anti-Synapsin I antibody used in Western Blot (WB). GTX82594

GTX82594 WB Image

Western blot of 10 ug of rat hippocampal (Hipp) lysate showing specific immunolabeling of the ~78k synapsin I doublet protein using Synapsin I antibody (GTX82594)

Anti-Synapsin I antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX82594

GTX82594 ICC/IF Image

ICC/IF analysis of cultured mouse caudate neurons showing punctate distribution using GTX82594 Synapsin I antibody.
Green : Primary antibody
Red : MAP antibody
Dilution : 1:1000

REFERENCE

Application Reference

Spagnuolo MS et al. Nutr Neurosci 2018; : 43112 Brain Nrf2 pathway, autophagy, and synaptic function proteins are modulated by a short-term fructose feeding in young and adult rats.
Application : WB
Reactivity : Rat
Chio CC et al. J Neuroinflammation 2017; 14 (1) : 90 Exercise attenuates neurological deficits by stimulating a critical HSP70/NF-κB/IL-6/synapsin I axis in traumatic brain injury rats.
Application : WB
Reactivity : Rat
Heyburn L et al. J Neurochem 2016; Tyrosine kinase inhibition reverses TDP-43 effects on synaptic protein expression, astrocytic function and amino acid dis-homeostasis.
Application : WB, IHC-Fr
Reactivity : Mouse
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Package List Price ($)
$ 499