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Synapsin II antibody

Anti-Synapsin II antibody used in Western Blot (WB). GTX13258

Cat No. GTX13258

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human, Mouse, Rat
Package
50 μl ($289)
APPLICATION

Application Note

WB: Use at a dilution of 1/5000. This dilution was sufficient for detection of Synapsin II in 15 μg of rat brain tissue extract. Detects bands of approximately 74 and 54 kDa, corresponding to the apparent molecular masses of synapsin IIa and IIb.(predicted molecular weight: 57.5 kDa). Optimal dilutions/concentrations should be determined by the end user.

Calculated MW

63 kDa. ( Note )

Positive Control

Rat brain tissue extract

Specificity/Sensitivity

Synapsin IIa and IIb.
PROPERTIES

Form

Liquid

Buffer

Preservative: NoneConstituents: Whole Serum

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

whole antiserum

Antigen Species

Rat

Immunogen

Synthetic peptide: ASPGPERRPPPAQA conjugated to KLH, corresponding to amino acids 52-65 of Rat Synapsin II. This sequence is identical in synapsin IIa and IIb.

Purification

Whole antiserum

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Synapsin Ii,Syn2

Cellular Localization

Synapse

Background

Synapsin II is a member of the synapsin gene family. Synapsins are neuronal phosphoproteins which associate with the cytoplasmic surface of synaptic vesicles. Family members are characterized by common protein domains, and they are implicated in synaptogenesis and the modulation of neurotransmitter release, suggesting a potential role in several neuropsychiatric diseases. This member of the synapsin family is a neuron-specific phosphoprotein that selectively binds to small synaptic vesicles in the presynaptic nerve terminal.

Database

Research Area

DATA IMAGES
Anti-Synapsin II antibody used in Western Blot (WB). GTX13258

GTX13258 WB Image

Western blot analysis of ArfGAP1 using 1) preimmune sera at 1:5000 in human heart and protein A purified GTX13258 in 2) human heart, 3) mouse heart and 4) rat heart at 2.0 ug/ml. A goat anti-rabbit Ig HRP secondary antibody and ECL substrate solution were used for this test.

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Package List Price ($)
$ 289